State Key Joint Laboratory of Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.
Folia Microbiol (Praha). 2020 Apr;65(2):315-322. doi: 10.1007/s12223-019-00726-y. Epub 2019 Jun 21.
Anaerobic ammonium-oxidizing (anammox) bacteria play an essential part in nitrogen removal in constructed wetlands. The objective of the present study was to explore the spatiotemporal dynamics of anammox bacterial communities and the associated factors in a full-scale constructed wetland for the treatment of polluted surface water. The abundance and diversity of anammox bacterial communities were characterized using quantitative polymerase chain reaction (PCR) and clone library analysis, respectively. Anammox bacterial diversity, richness, and abundance in the treatment wetland differed considerably among sampling sites and seasons, whereas anammox bacterial community structure tended to change slightly with site and time. Anammox abundance was likely influenced by temperature and the contents of nitrate and nitrite nitrogen. The increase of carbon and nitrogen contents could lower wetland anammox bacterial diversity and richness. Moreover, anammox bacterial diversity, richness, and abundance were also affected by wetland vegetation type. Candidatus Brocadia dominated in the treatment wetland, whereas Candidatus Kuenenia and a novel anammox phylotype were also detected. This work could provide some new insights towards anaerobic ammonium oxidization in surface water treatment wetland.
厌氧氨氧化（anammox）细菌在人工湿地脱氮过程中起着至关重要的作用。本研究的目的是探索用于处理受污染地表水的全尺寸人工湿地中厌氧氨氧化细菌群落的时空动态及其相关因素。分别采用定量聚合酶链反应（PCR）和克隆文库分析来描述厌氧氨氧化细菌群落的丰度和多样性。在处理湿地中，厌氧氨氧化细菌的多样性、丰富度和丰度在采样地点和季节之间存在显著差异，而厌氧氨氧化细菌群落结构随地点和时间的变化趋势较小。厌氧氨氧化菌丰度可能受温度、硝酸盐和亚硝酸盐氮含量的影响。碳氮含量的增加可能降低湿地厌氧氨氧化细菌的多样性和丰富度。此外，湿地植被类型也会影响厌氧氨氧化细菌的多样性、丰富度和丰度。Candidatus Brocadia 在处理湿地中占主导地位，而 Candidatus Kuenenia 和一种新型厌氧氨氧化菌也被检测到。这项工作可以为地表水处理湿地中的厌氧氨氧化提供一些新的见解。
