Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China.
University of Chinese Academy of Sciences, Beijing, China.
Microbiologyopen. 2018 Apr;7(2):e00556. doi: 10.1002/mbo3.556. Epub 2017 Dec 29.
This study investigated the diversity, community composition, and abundance of anaerobic ammonium oxidation (anammox) bacteria along the altitudinal gradient in Qinghai-Tibet Plateau. Two types of soil samples (wetland and dryland soils, n = 123) were collected from 641 m to 5,033 m altitudes. Polymerase chain reaction (PCR) screening showed that anammox were not widespread, and were only detected in 9 sampling sites of the 50 sites tested by amplifying the 16S rRNA genes. Then, only samples collected from Linzhi (2,715 m), Rikaze (4,030 m), and Naqu (5,011 m), which were positive for the presence of anammox, were further processed to explore the biogeography of anammox bacteria in Qinghai-Tibet Plateau. Results of high-throughput sequencing targeting the hydrazine synthesis β-subunit (hzsB) gene revealed the presence of three known anammox genera (Candidatus Brocadia, Candidatus Jettenia, and Candidatus Kuenenia) in both soil types. Their diversity, community composition, and abundance did not show significant variation with altitude at large scale. However, it was the small-scale environmental heterogeneities between wetland and dryland soils that determined their biogeographical distribution. Specifically, the dryland soils had higher diversity of anammox bacteria than the wetland soils, but their abundance patterns varied. The community composition of anammox bacteria were found to be influenced by soil nitrate content.
本研究调查了青藏高原海拔梯度上厌氧氨氧化（anammox）细菌的多样性、群落组成和丰度。从 641m 到 5033m 海拔高度采集了两种土壤样本（湿地土壤和旱地土壤，n=123）。聚合酶链反应（PCR）筛选表明，anammox 并不广泛，仅在 50 个测试点中的 9 个点通过扩增 16S rRNA 基因检测到。然后，仅对来自林芝（2715m）、日喀则（4030m）和那曲（5011m）的、存在 anammox 的样本进行进一步处理，以探索青藏高原 anammox 细菌的生物地理学。针对肼合成β-亚基（hzsB）基因的高通量测序结果表明，两种土壤类型中均存在三种已知的 anammox 属（Candidatus Brocadia、Candidatus Jettenia 和 Candidatus Kuenenia）。它们的多样性、群落组成和丰度在很大程度上没有随海拔的变化而显著变化。然而，是湿地和旱地土壤之间的小规模环境异质性决定了它们的生物地理分布。具体来说，旱地土壤中 anammox 细菌的多样性高于湿地土壤，但它们的丰度模式不同。发现 anammox 细菌的群落组成受土壤硝酸盐含量的影响。
