Gunin A G, Golubtzova N N
Chuvash State University, 15 Moscovskiy pr., Cheboksary 428015, Russian Federation; e-mail:
Adv Gerontol. 2019;32(1-2):12-19.
The aim of this work was to examine the content of transforming growth factor-β (TGF-β) in fibroblasts and blood microvessels of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of TGF-β in age-dependent changes in the number of fibroblasts and blood microvessels in the dermis. TGF-β, proliferating cells nuclear antigen (PCNA), endothelial cells marker CD 31 were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for TGF-β in the dermis is increased from 20 weeks of pregnancy until 85 years old. More expressed growth in percent of TGF-β positive fibroblasts in the human dermis is observed after birth and after 40 years old. The content of TGF-β in blood microvessels in the human dermis is decreased sufficiently from 41 years old. Age-related increase in a portion of fibroblasts with positive staining on TGF-β is associated with an age-related decrease in total number and percent of PCNA positive fibroblasts in human dermis. Age-related decrease in the content of TGF-β in blood microvessels is accompanied with an age-related decrease in their number in the dermis. Age-related increase in the content of TGF-β in fibroblasts is involved in an age-dependent decrease in their total number and proliferation in the dermis. Age-related decrease in the content of TGF-β in blood microvessels in dermis takes part in the age-related diminishing of blood microvessels number in the dermis.
这项工作的目的是研究从发育到深度衰老（从妊娠20周直到85岁）的人类真皮成纤维细胞和微血管中转化生长因子-β（TGF-β）的含量，并确定TGF-β在真皮成纤维细胞和微血管数量的年龄依赖性变化中的作用。采用间接免疫组织化学技术检测TGF-β、增殖细胞核抗原（PCNA）、内皮细胞标志物CD 31。结果显示，从妊娠20周直到85岁，真皮中TGF-β染色阳性的成纤维细胞比例增加。在出生后和40岁以后，观察到人类真皮中TGF-β阳性成纤维细胞百分比增长更为明显。从41岁起，人类真皮微血管中TGF-β的含量显著下降。真皮中TGF-β染色阳性的成纤维细胞比例随年龄增长而增加，这与人类真皮中PCNA阳性成纤维细胞总数和百分比随年龄增长而下降有关。真皮微血管中TGF-β含量随年龄增长而下降，同时其在真皮中的数量也随年龄增长而减少。真皮中TGF-β含量随年龄增长而增加，参与了真皮中其总数和增殖随年龄的减少。真皮微血管中TGF-β含量随年龄增长而下降，参与了真皮中微血管数量随年龄的减少。
