硫酸原黄素对转化脱氧核糖核酸产生损伤的特征
Features of the damage produced by proflavine on transforming deoxyribonucleic acid.
作者信息
Cabrera-Juárez E, Sánchez-Rincón D A
出版信息
J Bacteriol. 1979 Mar;137(3):1081-3. doi: 10.1128/jb.137.3.1081-1083.1979.
Proflavine formed a complex with transforming deoxyribonucleic acid (DNA) from Haemophilus influenzae, with optimal formation at a ratio of proflavine to DNA of 0.06. The rate of dissociation of the complex by dialysis increased in the order: native, denatured, renatured DNA. The transforming activity of the DNA was reduced by its interaction with proflavine. This inactivation was dependent on the physical state of the DNA, the proflavine concentration, and the temperature. DNA that had been denatured and renatured was most sensitive; native DNA was much less sensitive. The inactivation remained after dialysis and was stable to prolonged storage. It is concluded that the inactivation of transforming DNA by proflavine takes place by a mechanism different from that of DNA-proflavine complex formation.
原黄素与流感嗜血杆菌的转化脱氧核糖核酸（DNA）形成复合物，原黄素与DNA的比例为0.06时形成最佳复合物。通过透析使复合物解离的速率按以下顺序增加：天然DNA、变性DNA、复性DNA。DNA与原黄素相互作用会降低其转化活性。这种失活取决于DNA的物理状态、原黄素浓度和温度。变性后又复性的DNA最敏感；天然DNA则不太敏感。透析后失活仍然存在，并且在长时间储存后保持稳定。得出的结论是，原黄素使转化DNA失活的机制与DNA-原黄素复合物形成的机制不同。
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