Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243 122, UP, India.
Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar, 243 122, UP, India.
Eur J Pharmacol. 2019 Sep 5;858:172478. doi: 10.1016/j.ejphar.2019.172478. Epub 2019 Jun 20.
Nanoparticles have higher frequency of being exposed to cells or tissue, and are thus more likely to gain access into cytoplasm or nuclei to modulate molecular events due to significantly larger surface area to volume ratio. As a result, they present amplified response or even different physiochemical and biomedical properties from bigger particles. Deferoxamine accelerates wound healing in diabetic rats by increased neovascularization, reduced inflammation and improved maturation of wound. We investigated the wound healing potential of deferoxamine-nanoparticles in diabetic rats. Lecithin based nanoparticles of deferoxamine were prepared and characterized. The diabetic rats were divided into five Groups, of which Group I was treated with pluronic-gel f-127 (25%), Group II with deferoxamine 0.1% and Group III, IV and V were treated with deferoxamine-nanoparticles incorporated in pluronic-gel f-127 25% at 0.03% (0.01% deferoxamine), 0.1% (0.03% deferoxamine) and 0.3% (0.1% deferoxamine) w/v respectively. The wound closure was significantly accelerated in group V as compared to control groups. HIF-1α, VEGF, SDF-1α, TGF-β, and IL-10 protein levels were significantly higher in group V. The collagen deposition and neovascularization was greater in deferoxamine-nanoparticle treated rats. In contrast, TNF-α level was lowest in group V. In summary, the deferoxamine-nanoparticle formulation we developed, when applied topically on diabetic wounds results in faster wound healing as compared to simple deferoxamine formulation. This formulation may prove to be an effective therapy for treatment of diabetic wounds.
纳米颗粒更容易接触到细胞或组织，并且由于其更大的表面积与体积比，更有可能进入细胞质或细胞核来调节分子事件。因此，它们表现出放大的反应，甚至具有与较大颗粒不同的物理化学和生物医学特性。去铁胺通过增加新生血管形成、减少炎症和改善伤口成熟度来加速糖尿病大鼠的伤口愈合。我们研究了去铁胺纳米颗粒在糖尿病大鼠中的伤口愈合潜力。制备并表征了基于卵磷脂的去铁胺纳米颗粒。将糖尿病大鼠分为五组，其中 I 组用泊洛沙姆凝胶 f-127（25%）治疗，II 组用 0.1%去铁胺治疗，III、IV 和 V 组分别用 0.03%（0.01%去铁胺）、0.1%（0.03%去铁胺）和 0.3%（0.1%去铁胺）的泊洛沙姆凝胶 f-127 包载的去铁胺纳米颗粒治疗。与对照组相比，V 组的伤口闭合明显加快。V 组 HIF-1α、VEGF、SDF-1α、TGF-β 和 IL-10 蛋白水平显著升高。去铁胺纳米颗粒治疗的大鼠胶原沉积和新生血管形成更多。相比之下，V 组 TNF-α水平最低。总之，我们开发的去铁胺纳米颗粒制剂，与简单的去铁胺制剂相比，当局部应用于糖尿病伤口时，可更快地促进伤口愈合。这种制剂可能被证明是治疗糖尿病伤口的有效疗法。
