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球形红假单胞菌突变体从葡萄糖中以接近化学计量的量生成氢气。

Hydrogen formation in nearly stoichiometric amounts from glucose by a Rhodopseudomonas sphaeroides mutant.

作者信息

Macler B A, Pelroy R A, Bassham J A

出版信息

J Bacteriol. 1979 May;138(2):446-52. doi: 10.1128/jb.138.2.446-452.1979.

Abstract

Rhodopseudomonas sphaeroides produces molecular H2 and CO2 from reduced organic compounds which serve as electron sources and from light which provides energy in the form of adenosine 5'-triphosphate. This process is mediated by a nitrogenase enzyme. A mutant has been found that, unlike the wild type, will quantitatively convert glucose to H2 and CO2. Techniques for isolating other strains capable of utilizing other unusual electron sources are presented. Metabolism of glucose by the wild-type strain leads to an accumulation of gluconate. The isolated mutant strain does not appear to accumulate gluconate.

摘要

球形红假单胞菌利用还原态有机化合物作为电子源,并利用以腺苷5'-三磷酸形式提供能量的光,产生分子氢和二氧化碳。这个过程由固氮酶介导。已发现一种突变体,与野生型不同,它能将葡萄糖定量转化为氢气和二氧化碳。本文介绍了分离其他能够利用其他特殊电子源的菌株的技术。野生型菌株对葡萄糖的代谢会导致葡萄糖酸盐的积累。分离出的突变菌株似乎不会积累葡萄糖酸盐。

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