College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710119, China.
College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xi'an 710119, China; International Joint Research Center of Shaanxi Province for Food and Health Sciences, Xi'an 710119, China.
Int J Biol Macromol. 2019 Sep 15;137:504-511. doi: 10.1016/j.ijbiomac.2019.06.139. Epub 2019 Jun 20.
This work aims to analyze the immunomodulatory effect of pomegranate peel polysaccharides (PPP) on the immunosuppressed mice induced by cyclophosphamide (CTX). All the mice were divided into 6 groups randomly and the immunoprophylaxis mice were administrated with PPP [100, 200, 400 mg/(kg·d)] by gavage for consecutive 28 days. The results showed that PPP can slow down the decline of body weight and increase the immune organ index of the immunosuppressed mice. Compared to the model mice, the enzymatic activity of LDH (lactate dehydrogenase) and ACP (acid phosphatase) of the mice spleen administrated with PPP by gavage was enhanced significantly. PPP stimulated proliferation and secretion of splenic lymphocytes and markedly increased the immunoglobulin (Ig-A, Ig-G and Ig-M) expression and the release of cytokines (TNF-α, IL-2 and INF-γ) in cyclophosphamide-induced immunosuppressed mice. Hepatic antioxidant enzymatic activities of T-AOC (total antioxidant capacity), T-SOD (total superoxide dismutase), GSH-PX (glutathione peroxidase) and CAT (catalase) were markedly increased when the mice were administrated with high dosage of PPP. So it can be concluded that PPP could be used as an efficacious adjacent immunopotentiating therapy or an alternative means in lessening chemotherapy-induced immunosuppression, and also can be utilized as immunostimulants for food and pharmaceutical industries.
本研究旨在分析石榴皮多糖（PPP）对环磷酰胺（CTX）诱导的免疫抑制小鼠的免疫调节作用。所有小鼠随机分为 6 组，灌胃给予 PPP[100、200、400mg/（kg·d）]连续 28 天。结果表明，PPP 可减缓免疫抑制小鼠体重下降，增加免疫器官指数。与模型组相比，PPP 灌胃给药可显著增强小鼠脾组织中 LDH（乳酸脱氢酶）和 ACP（酸性磷酸酶）的酶活性。PPP 刺激脾淋巴细胞增殖和分泌，显著增加环磷酰胺诱导免疫抑制小鼠免疫球蛋白（Ig-A、Ig-G 和 Ig-M）的表达和细胞因子（TNF-α、IL-2 和 INF-γ）的释放。当小鼠给予高剂量 PPP 时，肝组织 T-AOC（总抗氧化能力）、T-SOD（总超氧化物歧化酶）、GSH-PX（谷胱甘肽过氧化物酶）和 CAT（过氧化氢酶）的抗氧化酶活性显著增加。综上所述，PPP 可作为一种有效的辅助免疫增强治疗或减轻化疗引起的免疫抑制的替代方法，也可作为食品和医药行业的免疫刺激剂。
