Zeng Yingnan, Yang Qi, Du Zhiyang, Liu Xuanting, Shang Xiaomin, Xu Menglei, Liu Jingbo, Lyu Siwen, Zhang Ting
Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, China.
Jilin Brewing Technology Innovation Center and College of Food Science and Nutritional Engineering, Jilin Agricultural Science and Technology University, Jilin 132101, China.
Foods. 2025 Jun 28;14(13):2295. doi: 10.3390/foods14132295.
Immunocompromise is a growing health concern, and food-derived immunomodulators are expected to serve as a valuable supplement to traditional drug therapies. Ovalbumin peptide (OP) was employed as a stabilizer to prepare OP-selenium nanoparticles (OP-SeNPs), which showed immunomodulatory effects in vitro; however, the effects and underlying mechanisms in vivo were not yet fully understood. This study investigated the immunomodulatory activity of OP-SeNPs in cyclophosphamide (CTX)-induced immunosuppressed mice on immune organs, molecules, and cells, with the underlying mechanism explored by transcriptomic and proteomic studies. The results demonstrated that OP-SeNPs alleviated tissue damage in the spleen and thymus, improved the immunosuppressive state by promoting the secretion of cytokines (IL-1β, IFN-γ, IL-4, and IL-6), immunoglobulins (IgA, IgG, IgM, and sIgA), and promoting the proliferation of splenic lymphocytes. PI3K-Akt, Rap1, p53, PPAR, and Hippo signaling pathways formed an important regulatory network that synergistically influenced immune modulation. OP-SeNPs are potential food-derived immunomodulators, setting the stage for deep exploration of the mechanisms driving their immunomodulatory effects.
免疫功能低下是一个日益严重的健康问题，食物来源的免疫调节剂有望成为传统药物治疗的宝贵补充。卵清蛋白肽（OP）被用作稳定剂来制备OP-硒纳米颗粒（OP-SeNPs），其在体外显示出免疫调节作用；然而，其在体内的作用及潜在机制尚未完全明确。本研究调查了OP-SeNPs对环磷酰胺（CTX）诱导的免疫抑制小鼠免疫器官、分子和细胞的免疫调节活性，并通过转录组学和蛋白质组学研究探索其潜在机制。结果表明，OP-SeNPs减轻了脾脏和胸腺的组织损伤，通过促进细胞因子（IL-1β、IFN-γ、IL-4和IL-6）、免疫球蛋白（IgA、IgG、IgM和sIgA）的分泌以及促进脾淋巴细胞增殖改善了免疫抑制状态。PI3K-Akt、Rap1、p53、PPAR和Hippo信号通路形成了一个重要的调控网络，协同影响免疫调节。OP-SeNPs是潜在的食物来源免疫调节剂，为深入探索其免疫调节作用机制奠定了基础。
