通过细胞荧光分析鉴定和定量B细胞和T细胞。

Identification and quantitation of B and T cells by cytofluorographic analysis.

作者信息

Beveridge R A, Super B S, Chretien P B

出版信息

J Immunol Methods. 1979;26(1):47-60. doi: 10.1016/0022-1759(79)90040-1.

Abstract

With cytofluorographic analysis (CFGA) of cells stained with the fluorescent dye acridine orange (AO), the major peripheral white cell populations--lymphocytes, monocytes, and polymorphonuclear cells--display different characteristics and appear as distinct populations which can be quantitated. In this study we present a method for determining percentages of human T and B cells lymphocyte subpopulations by CFGA and display of the data on a computer-generated 3-dimensional grid. Lymphocytes were depleted of either B, T, or both B and T cells by rosetting with erythrocytes and separated by centrifugation. The B cell and T cell depleted and non-rosetting cell subpopulations localized on constant, distinct areas of the display grid. The percentages of T and B cells in peripheral blood samples from 6 normals analyzed by CFGA did not differ from the results obtained by light microscope counting (LMC).

摘要

通过用荧光染料吖啶橙（AO）染色的细胞进行细胞荧光分析（CFGA），主要的外周白细胞群体——淋巴细胞、单核细胞和多形核细胞——呈现出不同的特征，并表现为可定量的不同群体。在本研究中，我们提出了一种通过CFGA以及在计算机生成的三维网格上展示数据来确定人T和B淋巴细胞亚群百分比的方法。通过与红细胞进行玫瑰花结形成使淋巴细胞去除B细胞、T细胞或同时去除B细胞和T细胞，然后通过离心分离。去除B细胞和T细胞以及未形成玫瑰花结的细胞亚群定位在展示网格的固定、不同区域。通过CFGA分析的6名正常人外周血样本中T细胞和B细胞的百分比与通过光学显微镜计数（LMC）获得的结果没有差异。