A technique for determining E rosette levels by cytofluorographic analysis.

A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish them from erythrocytes, and glutaraldehyde for fixation of the rosettes. This technique was compared with light microscope counting (LMC) or ER. CFGA gave similar results with better reproducibility than LMC, entailed less time for counting, and markedly reduced operator fatigue.