Goldberg N H, Kenady D E, Super B S, Chretien P B
J Immunol Methods. 1976;12(1-2):9-17. doi: 10.1016/0022-1759(76)90091-0.
A method is described for determining the percentage of rosettes formed by sheep erythrocytes and human peripheral lymphocytes (ER) using cytofluorographic analysis (CFGA). The technique utilizes acridine orange dye for differentially staining nucleus and cytoplasm of the lymphocytes to distinguish them from erythrocytes, and glutaraldehyde for fixation of the rosettes. This technique was compared with light microscope counting (LMC) or ER. CFGA gave similar results with better reproducibility than LMC, entailed less time for counting, and markedly reduced operator fatigue.
描述了一种使用细胞荧光分析（CFGA）测定绵羊红细胞与人类外周血淋巴细胞形成的玫瑰花结百分比的方法。该技术利用吖啶橙染料对淋巴细胞的细胞核和细胞质进行差异染色，以将它们与红细胞区分开来，并使用戊二醛固定玫瑰花结。将该技术与光学显微镜计数（LMC）测定玫瑰花结百分比的方法进行了比较。CFGA给出的结果与LMC相似，但重现性更好，计数所需时间更少，并且显著减轻了操作人员的疲劳。
