在免疫印迹分析中对检测ras p21高度敏感的单克隆抗体。
Monoclonal antibody highly sensitive for the detection of ras p21 in immunoblotting analysis.
作者信息
Kanai T, Hirohashi S, Noguchi M, Shimoyama Y, Shimosato Y, Noguchi S, Nishimura S, Abe O
机构信息
Pathology Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1987 Dec;78(12):1314-8.
Four murine monoclonal antibodies (NCC-RAS-001, -004, -005, -017) reactive with ras p21 were produced by using recombinant c-Ha-ras p21 as an immunogen. Among these antibodies, NCC-RAS-004 was extremely sensitive when used in immunoblotting analysis, facilitating semiquantitative detection of c-Ha-, c-Ki- and N-ras p21 in cell and tissue lysates. In cells carrying a point-mutationally activated ras, p21 with abnormal mobility upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis was clearly detected.
以重组c-Ha-ras p21作为免疫原，制备了4种与ras p21反应的鼠单克隆抗体（NCC-RAS-001、-004、-005、-017）。在这些抗体中，NCC-RAS-004用于免疫印迹分析时极为灵敏，有助于对细胞和组织裂解物中的c-Ha-、c-Ki-和N-ras p21进行半定量检测。在携带点突变激活型ras的细胞中，能清晰检测到在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中迁移率异常的p21。
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