College of Resource and Environment, Jilin Agricultural University, Changchun 130118, China.
College of Plant Sciences, Jilin University, Changchun 130062, China.
Int J Mol Sci. 2019 Jun 22;20(12):3053. doi: 10.3390/ijms20123053.
2N3 is a strain of gram-negative bacteria that can degrade chlorimuron-ethyl and grow with chlorimuron-ethyl as the sole nitrogen source. The complete genome of 2N3 was sequenced using third generation high-throughput DNA sequencing technology. The genomic size of strain 2N3 was 5.32 Mb with a GC content of 57.33% and a total of 5156 coding genes and 112 non-coding RNAs predicted. Two hydrolases expressed by open reading frames (ORFs) 0934 and 0492 were predicted and experimentally confirmed by gene knockout to be involved in the degradation of chlorimuron-ethyl. Strains of ΔORF 0934, ΔORF 0492, and wild type (WT) reached their highest growth rates after 8-10 hours in incubation. The degradation rates of chlorimuron-ethyl by both ΔORF 0934 and ΔORF 0492 decreased in comparison to the WT during the first 8 hours in culture by 25.60% and 24.74%, respectively, while strains ΔORF 0934, ΔORF 0492, and the WT reached the highest degradation rates of chlorimuron-ethyl in 36 hours of 74.56%, 90.53%, and 95.06%, respectively. This study provides scientific evidence to support the application of 2N3 in bioremediation to control environmental pollution.
2N3 是一株能够降解氯嘧磺隆-乙基且以氯嘧磺隆-乙基为唯一氮源生长的革兰氏阴性细菌。使用第三代高通量 DNA 测序技术对 2N3 的全基因组进行了测序。菌株 2N3 的基因组大小为 5.32Mb，GC 含量为 57.33%，共预测出 5156 个编码基因和 112 个非编码 RNA。通过基因敲除预测并实验证实，开放阅读框（ORF）0934 和 0492 表达的两种水解酶参与了氯嘧磺隆-乙基的降解。ΔORF 0934、ΔORF 0492 和野生型（WT）菌株在孵育 8-10 小时后达到最高生长速率。与 WT 相比，ΔORF 0934 和 ΔORF 0492 在培养的前 8 小时内，氯嘧磺隆-乙基的降解率分别下降了 25.60%和 24.74%，而 ΔORF 0934、ΔORF 0492 和 WT 菌株在 36 小时内达到最高氯嘧磺隆-乙基降解率分别为 74.56%、90.53%和 95.06%。本研究为 2N3 在生物修复控制环境污染中的应用提供了科学依据。
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