Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
QJM. 2019 Oct 1;112(10):779-785. doi: 10.1093/qjmed/hcz158.
Recently, much attention has been paid to use circulating microRNAs (miRs) as a non-invasive tumor marker. The present study for the first time was designed to evaluate concurrent use of miR-21, miR-638, miR148 and miR-152 as putative diagnostic tool for detection of non-small cell lung carcinoma (NSCLC).
Forty-three patients diagnosed as primary NSCLC was included in this study. The level of selected miRs was measured in whole blood specimens of patients and controls. The corresponding values were also obtained in stages I-IV. We also assessed possible correlation between selected miRs and the clinicopathological findings of studied individuals.
miR-21 was increased in patients compared to controls (P = 0.004). In contrast, circulating miR-638, miR-148 and miR-152 was observed to be down-regulated in NSCLC patients than controls (P = 0.001, 0.003, 0.053, respectively). Rise in miR-21-5p expression and decreased blood level of miR-148a-3p was associated with higher stage of NSCLC. The highest sensitivity (90%) was observed for miR-21 while miR-148 had the highest specificity (71%). The corresponding sensitivity and specificity for combined-miRs-panel was 96.4% and 86.67%, respectively.
In summary, our data suggested the diagnostic importance of combined-miR-panel including miR-21, miR-638, miR148 and miR-152 for effective discrimination of NSCLC from non-cancerous subjects.
最近，人们越来越关注将循环 microRNAs（miRs）用作非侵入性肿瘤标志物。本研究首次旨在评估 miR-21、miR-638、miR148 和 miR-152 联合作为非小细胞肺癌（NSCLC）检测的潜在诊断工具。
本研究纳入了 43 名被诊断为原发性 NSCLC 的患者。在患者和对照组的全血标本中测量了选定 miRs 的水平。还在 I-IV 期获得了相应的值。我们还评估了选定的 miRs 与研究个体的临床病理发现之间的可能相关性。
与对照组相比，患者的 miR-21 升高（P=0.004）。相比之下，在 NSCLC 患者中观察到循环 miR-638、miR-148 和 miR-152 的水平下调（P=0.001、0.003 和 0.053）。miR-21-5p 表达的升高和 miR-148a-3p 血水平的降低与 NSCLC 的较高分期相关。miR-21 的敏感性最高（90%），而 miR-148 的特异性最高（71%）。联合-miRs 谱的敏感性和特异性分别为 96.4%和 86.67%。
总之，我们的数据表明，联合-miR 谱（包括 miR-21、miR-638、miR148 和 miR-152）对于有效区分 NSCLC 与非癌患者具有重要的诊断意义。
