Kovtunenko L V, Bukrinskiĭ M I, Il'iashenko B N
Mol Gen Mikrobiol Virusol. 1987 Oct(10):27-30.
The process of polyethyleneglycol-induced plasmid transformation of Bacillus cereus protoplasts was studied. Plasmid transfer into Bacillus cereus strains was demonstrated with the frequencies 1.3.10(1)-1.6.10(2) transformants per 1 mkg of plasmid DNA. The plasmids transferred are stably inherited by Bacillus cereus cells causing tetracycline resistance (pBC16) or kanamycin resistance (pUB110 and pBD64). The proposed method can be used for construction of Bacillus cereus strains having the plasmid determined characteristics.
研究了聚乙二醇诱导蜡样芽孢杆菌原生质体进行质粒转化的过程。已证实质粒可转入蜡样芽孢杆菌菌株,转化频率为每1微克质粒DNA产生1.3×10¹ - 1.6×10²个转化子。转入的质粒可被蜡样芽孢杆菌细胞稳定遗传,赋予其四环素抗性(pBC16)或卡那霉素抗性(pUB110和pBD64)。所提出的方法可用于构建具有质粒确定特性的蜡样芽孢杆菌菌株。