质粒pBC16介导的苏云金芽孢杆菌蜡螟亚种原生质体转化
Transformation of Bacillus thuringiensis subsp. galleria protoplasts by plasmid pBC16.
作者信息
Alikhanian S I, Ryabchenko N F, Bukanov N O, Sakanyan V A
出版信息
J Bacteriol. 1981 Apr;146(1):7-9. doi: 10.1128/jb.146.1.7-9.1981.
Abstract
Protoplasts of the entomopathogenic bacterium Bacillus thuringiensis subsp. galleria were transformed by plasmid pBC16. The frequency of transformation was much lower than that of Bacillus subtilis. All isolated B. thuringiensis transformants were characterized by increased sensitivity to lysozyme as compared with the original strain.
摘要
昆虫病原细菌苏云金芽孢杆菌蜡螟亚种的原生质体用质粒pBC16进行转化。转化频率远低于枯草芽孢杆菌。与原始菌株相比，所有分离得到的苏云金芽孢杆菌转化子的特征是对溶菌酶的敏感性增加。
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