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质粒pBC16介导的苏云金芽孢杆菌蜡螟亚种原生质体转化

Transformation of Bacillus thuringiensis subsp. galleria protoplasts by plasmid pBC16.

作者信息

Alikhanian S I, Ryabchenko N F, Bukanov N O, Sakanyan V A

出版信息

J Bacteriol. 1981 Apr;146(1):7-9. doi: 10.1128/jb.146.1.7-9.1981.

DOI:10.1128/jb.146.1.7-9.1981
PMID:7217007
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217044/
Abstract

Protoplasts of the entomopathogenic bacterium Bacillus thuringiensis subsp. galleria were transformed by plasmid pBC16. The frequency of transformation was much lower than that of Bacillus subtilis. All isolated B. thuringiensis transformants were characterized by increased sensitivity to lysozyme as compared with the original strain.

摘要

昆虫病原细菌苏云金芽孢杆菌蜡螟亚种的原生质体用质粒pBC16进行转化。转化频率远低于枯草芽孢杆菌。与原始菌株相比，所有分离得到的苏云金芽孢杆菌转化子的特征是对溶菌酶的敏感性增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d38/217044/15d4f839f9ef/jbacter00269-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d38/217044/15d4f839f9ef/jbacter00269-0018-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d38/217044/15d4f839f9ef/jbacter00269-0018-a.jpg

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本文引用的文献

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Chemical composition of the cell wall of Bacillus thuringiensis var. thuringiensis.苏云金芽孢杆菌苏云金变种细胞壁的化学成分。
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苏云金芽孢杆菌营养细胞通过质粒DNA进行转化。
J Bacteriol. 1987 Mar;169(3):1147-52. doi: 10.1128/jb.169.3.1147-1152.1987.
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Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.通过转化导入枯草芽孢杆菌的金黄色葡萄球菌质粒的特性分析。
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Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis.蜡样芽孢杆菌和枯草芽孢杆菌中的细菌素与抗生素抗性质粒
J Bacteriol. 1978 Feb;133(2):897-903. doi: 10.1128/jb.133.2.897-903.1978.
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[Isolation of nontransmissible deletion variants and the genetic map of pAS8 plasmid (RP4-ColE1)].[非传递性缺失变体的分离及pAS8质粒（RP4-ColE1）的遗传图谱]
Genetika. 1977;13(10):1778-89.
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High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.利用质粒DNA对枯草芽孢杆菌原生质体进行高频转化。
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