Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standards & Testing Technology for Agro-products , Chinese Academy of Agricultural Sciences , Beijing 100081 , People's Republic of China.
J Agric Food Chem. 2019 Jun 26;67(25):7167-7173. doi: 10.1021/acs.jafc.9b01078. Epub 2019 Jun 17.
An improved method for solid-phase extraction (SPE)-liquid chromatography-tandem mass spectrometry was developed to analyze eight monoalkyl phthalate esters (MPEs) in eight different kinds of porcine tissues. The tissue samples were processed using enzymatic deconjugation with β-glucuronidase, followed by SPE with Oasis MAX cartridges. A pentafluorophenyl column was first used to solve the coeluting issues of MPE isomers. The limits of detection and recoveries were 0.01-0.6 ng/g and 62.5-123.7%, respectively. The intra- and interday precisions were less than 7.1 and 9.4%, respectively. The robust method was successfully applied for the investigation of MPEs in various porcine tissue samples collected from markets in Beijing, China. The occurrence of MPEs with total concentrations of 48.0-108 ng/g was detected, and monoethylhexyl phthalate was the predominant MPE (accounting for 30-57%) in all of the porcine tissue samples. The results will be helpful in assessing the potential risks of diets that include pork.
建立了一种改进的固相萃取（SPE）-液相色谱-串联质谱法，用于分析八种不同猪组织中的八种单烷基邻苯二甲酸酯（MPEs）。组织样品先用β-葡萄糖醛酸酶进行酶解去结合，然后用 Oasis MAX 小柱进行 SPE 处理。首先使用五氟苯基柱解决 MPE 异构体的共洗脱问题。检测限和回收率分别为 0.01-0.6ng/g 和 62.5-123.7%。日内和日间精密度分别小于 7.1%和 9.4%。该稳健的方法成功应用于检测中国北京市场采集的各种猪组织样品中的 MPEs。检测到 MPEs 的总浓度为 48.0-108ng/g，并且在所有猪组织样品中，邻苯二甲酸单乙基己基酯是主要的 MPE（占 30-57%）。这些结果将有助于评估包括猪肉在内的饮食的潜在风险。
