Gvozdjáková Anna, Kucharská Jarmila, Sumbalová Zuzana, Nemec Michal, Chládeková Anežka, Vančová Oľga, Rausová Zuzana, Kubalová Mária, Kuzmiaková Zuzana, Mojto Viliam
Pharmacobiochemical Laboratory of 3rd Medical Department, Medical Faculty, Comenius University in Bratislava, Bratislava, Slovakia.
Institute of Experimental Endocrinology, Biomedical Research Center Slovak Academy of Sciences, Bratislava, Slovakia.
Gen Physiol Biophys. 2019 Jul;38(4):325-334. doi: 10.4149/gpb_2019012. Epub 2019 Jun 26.
Seasonal variations in temperature may influence the physiological and pathological metabolic pathways, concentrations of antioxidants, degree of oxidative stress and mitochondrial function. The aim of this study was to evaluate platelet mitochondrial function in human subjects during seasonal variations in temperature. Two groups of healthy young subjects were enroled in the study. Winter group, mean outside temperature was 4.77°C and Spring group, mean outside temperature was 24.32°C. High-resolution respirometry method was used for determination of mitochondrial respiration and oxidative phosphorylation in platelets. Concentrations of coenzyme Q10 (CoQ10) and tocopherols were determined in platelets, blood and plasma. Our data showed slightly (not significantly) reduced respiration in intact platelets, basal and ADP-stimulated mitochondrial respiration at Complex I, as well as CoQ10-TOTAL and α-tocopherol concentrations in winter. The concentration of γ-tocopherol was higher in winter. Platelet mitochondrial ATP production depended on platelet CoQ10-TOTAL concentration in winter, not in spring. We conclude that seasonal temperature participates in the mechanism of platelet mitochondrial respiratory chain function and oxidative phosphorylation that depends on their CoQ10-TOTAL concentration at lower winter outside temperature. CoQ10 supplementation may improve platelets mitochondrial ATP production at winter season. High-resolution respirometry offers sensitive method for detection of changes of platelets mitochondrial respiratory function.
温度的季节性变化可能会影响生理和病理代谢途径、抗氧化剂浓度、氧化应激程度和线粒体功能。本研究的目的是评估人体在温度季节性变化期间血小板的线粒体功能。两组健康年轻受试者参与了该研究。冬季组，平均室外温度为4.77°C，春季组，平均室外温度为24.32°C。采用高分辨率呼吸测定法测定血小板中的线粒体呼吸和氧化磷酸化。测定了血小板、血液和血浆中辅酶Q10（CoQ10）和生育酚的浓度。我们的数据显示，冬季完整血小板的呼吸、复合体I处基础和ADP刺激的线粒体呼吸略有（不显著）降低，以及CoQ10总量和α-生育酚浓度。冬季γ-生育酚浓度较高。冬季血小板线粒体ATP生成取决于血小板CoQ10总量浓度，而春季则不然。我们得出结论，季节性温度参与了血小板线粒体呼吸链功能和氧化磷酸化机制，这取决于冬季较低室外温度下它们的CoQ10总量浓度。补充CoQ10可能会改善冬季血小板线粒体ATP生成。高分辨率呼吸测定法为检测血小板线粒体呼吸功能变化提供了灵敏的方法。
