Li Junni, Lin Xiaojing, Tang Gengqiu, Li Rong, Wang Dong, Ji Shengguo
School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, 280, Outer Ring Road East, Higher Education Mega Center, 510006 Guangdong, PR, China.
An Acad Bras Cienc. 2019 Jun 19;91(2):e20180637. doi: 10.1590/0001-3765201920180637.
Desmodium caudatum (Thunb.) DC, is an ever-green plant widely used in the central and southern China with great economic value for their medical values on fever, dysentery, gastroenteritis, rectal prolapse, snake bites, mastitis, and boils carbuncle. Despite its extensive uses as a traditional Chinese medicine, no systematic research on the identification of Desmodium caudatum has been reported. In this study, traditional pharmacognostical identification including the botanical origin and morphological characters, medicinal material characters, microscopic characters, physicochemical parameters determination and phytochemical screening, and DNA barcoding analysis were employed to establish an accurate and effective identification system of Desmodium caudatum. In addition, the molecular pharmacognosy study was adopted in order to identify the samples more accurately. The ITS loci of the nuclear genome and psbA-trnH loci of the chloroplast genome were selected and evaluated, which were the most variable loci. The study will be beneficial to the development of the quality standard and the identification of species.
长柄山蚂蝗（Desmodium caudatum (Thunb.) DC）是一种常绿植物，在中国中部和南部广泛种植，因其对发热、痢疾、肠胃炎、直肠脱垂、蛇咬伤、乳腺炎和疔疮痈肿具有药用价值而具有很高的经济价值。尽管它作为一种传统中药被广泛使用，但尚未有关于长柄山蚂蝗鉴定的系统研究报道。在本研究中，采用了传统生药学鉴定方法，包括植物来源和形态特征、药材性状、显微特征、理化参数测定和植物化学筛选，以及DNA条形码分析，以建立一个准确有效的长柄山蚂蝗鉴定系统。此外，采用分子生药学研究以便更准确地鉴定样品。选择并评估了核基因组的ITS位点和叶绿体基因组的psbA-trnH位点，它们是变异最大的位点。该研究将有助于质量标准的制定和物种鉴定。
