Department of Chemistry, Centre for Biotechnology , Brock University , 1812 Sir Isaac Brock Way , St. Catharines , Ontario , Canada L2S 3A1.
College of Chemistry, Analytical & Testing Centre , Sichuan University , Chengdu 610064 , China.
ACS Nano. 2019 Jul 23;13(7):8106-8113. doi: 10.1021/acsnano.9b03053. Epub 2019 Jun 26.
Herein, we report a bottom-up approach to assemble a series of stochastic DNA walkers capable of probing dynamic interactions occurring at the bio-nano interface. We systematically investigated the impact of varying interfacial factors, including intramolecular interactions, orientation, cooperativity, steric effect, multivalence, and binding hindrance on enzymatic behaviors at the interfaces of spherical nucleic acids. Our mechanistic study has revealed critical roles of various interfacial factors that significantly alter molecular binding and enzymatic behaviors from bulk solutions. The improved understanding of the bio-nano interface may facilitate better design and operation of nanoparticle-based biosensors and/or functional devices. We successfully demonstrate how improved understanding of the bio-nano interface help rationalize the design of amplifiable biosensors for nucleic acids and antibodies.
在这里，我们报告了一种自下而上的方法，用于组装一系列能够探测生物-纳米界面上动态相互作用的随机 DNA 行走者。我们系统地研究了界面因素的变化对球形核酸界面上酶行为的影响，包括分子内相互作用、取向、协同作用、空间位阻、多价和结合阻碍。我们的机制研究揭示了各种界面因素的关键作用，这些因素显著改变了分子结合和从体相溶液中的酶行为。对生物-纳米界面的更好理解可能有助于更好地设计基于纳米粒子的生物传感器和/或功能器件，并使其更好地运行。我们成功地证明了如何通过更好地理解生物-纳米界面来帮助合理化用于核酸和抗体的可扩增生物传感器的设计。
