Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China.
State Key Laboratory of Food Nutrition and Safety, Tianjin Engineering Research Center of Safety Control Technology in Food Processing, Tianjin, 300457, China.
Anal Bioanal Chem. 2019 Sep;411(22):5745-5753. doi: 10.1007/s00216-019-01954-4. Epub 2019 Jun 26.
A label-free piezoelectric immunosensor was fabricated and applied to the detection of the antiviral drug amantadine (AM) in foods of animal origin. Experimental parameters associated with the fabrication and measurement process were optimized and are discussed here in detail. The proposed piezoelectric sensor is based on an immunosuppression format and uses a portable quartz crystal microbalance (QCM) chip. It was found to provide a good response to AM, with a sensitivity and limit of detection (LOD) of 33.9 and 1.3 ng mL, respectively, as well as low cross-reactivity (CR, < 0.01%) with AM analogues. The immunosensor was further applied to quantify AM at three levels in spiked samples of typical foods of animal origin, and yielded recoveries of 83.2-93.4% and standard deviations (SDs, n = 3) of 2.4-4.5%, which are comparable to the results (recoveries: 82.6-94.3%; SDs: 1.7-4.2%) obtained using a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method. Furthermore, the piezoelectric immunosensing chip can be regenerated multiple (at least 20) times with low signal attenuation (about 10%). A sample analysis can be completed within 50 min (sample pretreatment: about 40 min, QCM measurement: 5 min). These results demonstrate that the developed piezoelectric immunosensor provides a sensitive, accurate, portable, and low-cost analytical strategy for the antiviral drug AM in foods of animal origin, and this label-free detection method could also be applied to analyze other targets in the field of food safety. Graphical abstract.
一种无标记压电免疫传感器被构建并应用于检测动物源食品中的抗病毒药物金刚烷胺 (AM)。与制备和测量过程相关的实验参数进行了优化，并在此详细讨论。所提出的压电传感器基于免疫抑制格式，并使用便携式石英晶体微天平 (QCM) 芯片。结果表明，该传感器对 AM 具有良好的响应，灵敏度和检测限 (LOD) 分别为 33.9 和 1.3ng mL，与 AM 类似物的交叉反应性 (CR) 低 (<0.01%)。该免疫传感器进一步应用于定量分析动物源典型食品中加标样品中的 AM，回收率为 83.2-93.4%，标准偏差 (SD，n=3) 为 2.4-4.5%，与高效液相色谱-串联质谱法 (HPLC-MS/MS) 方法的结果 (回收率：82.6-94.3%；SDs：1.7-4.2%) 相当。此外，压电免疫传感芯片可以在信号衰减约 10%的情况下进行至少 20 次再生。样品分析可在 50 分钟内完成(样品预处理：约 40 分钟，QCM 测量：5 分钟)。这些结果表明，所开发的压电免疫传感器为动物源食品中的抗病毒药物 AM 提供了一种灵敏、准确、便携且低成本的分析策略，这种无标记检测方法也可应用于食品安全领域中分析其他目标。
