Shcherbakova N S, Chikaev A N, Rudometov A P, Shcherbakov D N, Il'ichev A A, Karpenko L I
State Research Center of Virology and Biotechnology Vector, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being (Rospotrebnadzor), Kol'tsovo, Novosibirsk region, Russia.
Institute of Molecular and Cellular Biology, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Bull Exp Biol Med. 2019 Jun;167(2):259-262. doi: 10.1007/s10517-019-04504-1. Epub 2019 Jun 26.
The paper describes construction of TBI-based recombinant proteins TBI-2F5 and TBI-2G12 that contain peptide mimotopes of HIV-1 epitopes recognized by broadly neutralizing antibodies 2F5 and 2G12, respectively. The capacity of the immunogens to induce neutralizing antibodies was evaluated. The sera of BALB/c mice immunized with recombinant proteins TBI, TBI-2F5, and TBI-2G12 neutralized HIV-1 env-pseudoviruses. Moreover, pooled serum from mice immunized with TBI-2F5 and TBI-2G12 neutralized env-pseudoviruses of HIV-1 subtype B more effectively than individual sera.
该论文描述了基于TBI的重组蛋白TBI-2F5和TBI-2G12的构建，它们分别包含被广泛中和抗体2F5和2G12识别的HIV-1表位的肽模拟表位。评估了免疫原诱导中和抗体的能力。用重组蛋白TBI、TBI-2F5和TBI-2G12免疫的BALB/c小鼠血清可中和HIV-1 env假病毒。此外，用TBI-2F5和TBI-2G12免疫的小鼠的混合血清比单个血清更有效地中和HIV-1 B亚型的env假病毒。
