Jain Jyoti, Legan Susan K, Alhamoud Issa, Gattineni Jyothsna, Baum Michel
Department of Pediatrics, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas.
Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas.
Physiol Rep. 2019 Jul;7(12):e14154. doi: 10.14814/phy2.14154.
We have previously demonstrated that dexamethasone administered to pregnant rats during specific times during gestation results in a reduction in glomerular number and hypertension in offspring at 2 and 6 months of age. In this study, we examined the effect of prenatal dexamethasone administered daily on days 15 and 16 of gestation in male and female offspring after 1 year of age on glomerular filtration rate. The prenatal dexamethasone male group had a higher systolic blood pressure than the vehicle male group. Females had lower systolic blood pressures than the males and prenatal dexamethasone did not affect blood pressure in female offspring. Prenatal dexamethasone resulted in a reduction in glomerular filtration rate in male but not in female rats. When corrected for body weight, the control male rats had a lower glomerular filtration rate than the control female rats. Males had greater protein excretion than females and prenatal dexamethasone increased the protein excretion only in male rats. Glomerulosclerosis was also greater in male rats than females but was not affected by prenatal dexamethasone. In summary, male rats appear to have evidence of a decline in glomerular filtration rate after 1 year of age and prenatal dexamethasone programs an accelerated decline in glomerular filtration rate in male but not in female offspring.
我们之前已经证明，在孕期特定时间给怀孕大鼠注射地塞米松，会导致其后代在2个月和6个月大时肾小球数量减少和出现高血压。在本研究中，我们检测了在妊娠第15天和第16天每天注射产前地塞米松，对1岁后雄性和雌性后代肾小球滤过率的影响。产前地塞米松雄性组的收缩压高于溶剂对照组雄性组。雌性的收缩压低于雄性，且产前地塞米松不影响雌性后代的血压。产前地塞米松导致雄性大鼠而非雌性大鼠的肾小球滤过率降低。校正体重后，对照雄性大鼠的肾小球滤过率低于对照雌性大鼠。雄性的蛋白质排泄量高于雌性，且产前地塞米松仅增加雄性大鼠的蛋白质排泄量。雄性大鼠的肾小球硬化也比雌性更严重，但不受产前地塞米松影响。总之，雄性大鼠在1岁后似乎有肾小球滤过率下降的迹象，产前地塞米松使雄性后代而非雌性后代的肾小球滤过率加速下降。
