Reed Research Group, Department of Animal Sciences, University of Wisconsin-Madison, 1675 Observatory Drive, Madison, Wisconsin 53706, United States.
National Center for Biotechnological Innovations of Costa Rica (CENIBiot), 1174-1200 San José, Costa Rica.
J Agric Food Chem. 2020 Mar 11;68(10):2872-2879. doi: 10.1021/acs.jafc.9b01451. Epub 2019 Jun 17.
Total polyphenol content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), and proanthocyanidin (PAC) content were determined in fruit from three tropical species (, , and ) from Costa Rica sampled at three stages of fruit development. Results show that TAC increased as the fruit developed, while TPC, TFC, and PAC content decreased. Anthocyanin profiles were evaluated using electrospray ionization tandem mass spectrometry. Cyanidin and delphinidin glycosides were the predominant anthocyanins for the three tropical species. Proanthocyanidins were characterized using attenuated total reflection Fourier transform infrared spectroscopy, nuclear magnetic resonance, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The presence of procyanidin structures with B-type interflavan bonds were observed, but deconvolution of mass spectrometry isotope patterns indicated that PACs with one or more A-type interflavan bonds accounted for more than 74% of the oligomers at each degree of polymerization.
总多酚含量（TPC）、总黄酮含量（TFC）、总花色苷含量（TAC）和原花青素（PAC）含量在哥斯达黎加三种热带水果（、和）的果实中进行了测定，这些水果分别在果实发育的三个阶段进行了采样。结果表明，随着果实的发育，TAC 增加，而 TPC、TFC 和 PAC 含量下降。采用电喷雾串联质谱法评价了花色苷的分布。对于这三种热带水果，矢车菊素和飞燕草素糖苷是主要的花色苷。采用衰减全反射傅里叶变换红外光谱、核磁共振和基质辅助激光解吸/电离飞行时间质谱对原花青素进行了表征。观察到存在 B 型间黄烷键的原花青素结构，但质谱同位素模式的解卷积表明，在每个聚合度下，具有一个或多个 A 型间黄烷键的 PAC 占低聚物的比例超过 74%。
