Huang Hsiang-Ming, Ho Chien-Yi, Chang Geng-Ruei, Shia Wei-Yau, Lai Cheng-Hung, Chao Chih-Hao, Wang Chao-Min
Neurosurgery Department, China Medical University Hsinchu Hospital, Hsinchu 302, Taiwan.
Department of Biomedical Imaging and Radiological Science, China Medical University, Taichung 404, Taiwan.
Pharmaceuticals (Basel). 2021 Oct 28;14(11):1098. doi: 10.3390/ph14111098.
Hayata is a medicinal plant that has been historically used in ethnopharmacy to treat diseases in Taiwan. The objective of this study is to evaluate the anti-cancer and anti-bacterial constitutes from the root nodule extract of . The chemical composition of fractions was analyzed by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) and the chemical constitutes were isolated and structurally identified by nuclear magnetic resonance (NMR) spectroscopy. Bioassay-guided chromatography showed that the ethyl acetate (EA) fraction was bioactive on the hepatocellular carcinoma (HepG2). By LC-ESI-MS/MS analysis, twenty peaks of EA fraction were partially identified and the phytochemical investigation of the fractions led to the isolation and identification of protocatuchuic acid (), epicatechin (), catechin (), procyanidin B3 (), procyanidin A1 (), hyperin (), isoquercetin (), quercetin (), lupeol (), beta-amyrin (), and alpha-amyrin (). Both procyanidin B3 and A1 exhibited anti-proliferative activity against HepG2 and gastric adenocarcinoma (AGS) cells at IC values between 38.4 and 41.1 μM and 79.4 and 83.8 μM, respectively. In addition, isoquercetin displayed the strongest anti-proliferative activity against the HepG2, lung carcinoma (A549), and AGS cell at 18.7, 24.6 and 68.5 μM, respectively. Among the triterpenoids, only lupeol showed the inhibitory activity against all tested tumor cell lines at IC values between 72.9 and 146.8 μM. Furthermore, procyanidins B3, A1 and isoquercetin displayed moderate anti-bacterial activity against . In conclusion, this study provides background information on the exploitation of as a potential natural anti-cancer and anti-bacterial agent in pharmaceutical research.
早田氏泽兰是一种药用植物，在台湾的民族药学中，长期以来一直被用于治疗疾病。本研究的目的是评估早田氏泽兰根瘤提取物中的抗癌和抗菌成分。通过高效液相色谱-电喷雾电离串联质谱法（HPLC-ESI-MS/MS）分析各馏分的化学成分，并通过核磁共振（NMR）光谱法分离和鉴定化学成分。生物活性导向色谱分析表明，乙酸乙酯（EA）馏分对肝癌细胞（HepG2）具有生物活性。通过LC-ESI-MS/MS分析，部分鉴定了EA馏分的20个峰，并对这些馏分进行了植物化学研究，从而分离和鉴定了原儿茶酸、表儿茶素、儿茶素、原花青素B3、原花青素A1、金丝桃苷、异槲皮苷、槲皮素、羽扇豆醇、β-香树脂醇和α-香树脂醇。原花青素B3和A1对HepG2和胃腺癌细胞（AGS）均具有抗增殖活性，其IC值分别在38.4至41.1 μM和79.4至83.8 μM之间。此外，异槲皮苷对HepG2、肺癌细胞（A549）和AGS细胞的抗增殖活性最强，IC值分别为18.7、24.6和68.5 μM。在三萜类化合物中，只有羽扇豆醇对所有测试的肿瘤细胞系具有抑制活性，IC值在72.9至146.8 μM之间。此外，原花青素B3、A1和异槲皮苷对[具体细菌名称未给出]显示出中等抗菌活性。总之，本研究为在药物研究中开发早田氏泽兰作为潜在的天然抗癌和抗菌剂提供了背景信息。
