Nourmohammadi Bahareh, Tafsiri Elham, Rahimi Amirabbas, Nourmohammadi Zahra, Daneshvar Kakhaki Abolghasem, Cho William, Karimipoor Morteza
Department of Molecular Medicine, Biotechnology Research center, Pasteur Institute of Iran, Tehran, Iran. Email:
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Asian Pac J Cancer Prev. 2019 Jun 1;20(6):1633-1639. doi: 10.31557/APJCP.2019.20.6.1633.
MicroRNAs (miRNAs) exert a critical influence on physiological and pathological processes through posttranscriptional modification of their mRNA targets. They play important roles in tumorigenesis and are considered to be potential diagnostic and prognostic biomarkers with various cancers. MiR-200c and miR-9 are regulatory elements that can have dual impacts as oncogenes and/or tumor suppressor genes. MiR-200c regulates two transcription factors, ZEB1 and ZEB2, while miR-9 is a regulatory factor for the E-cadherin protein which has a critical function in cell-cell junctions and is inhibited by two transcription factors ZEB1 and ZEB2. In this study, expression levels of miR-200c and miR-9, ZEB-1, ZEB-2 and E-cadherin were assessed in 30 non-small cell lung cancers (NSCLCs) by real-time qPCR. MiR-9 was down-regulated significantly in tumor tissues compared to normal adjacent tissues, while there was no significant change in expression level of miR-200c. On the other hand, ZEB1 demonstrated significant increase and ZEB2a decrease at the mRNA level. These results indicate roles for miR-9 and ZEB1 in genesis of lung cancer, although clinico-pathological associations were not evident. Further studies are necessary to assess implications for treatment of lung cancer.
微小RNA（miRNA）通过对其mRNA靶标的转录后修饰，对生理和病理过程产生关键影响。它们在肿瘤发生过程中发挥重要作用，被认为是多种癌症潜在的诊断和预后生物标志物。MiR-200c和miR-9是调控元件，可作为癌基因和/或肿瘤抑制基因产生双重影响。MiR-200c调控两种转录因子ZEB1和ZEB2，而miR-9是E-钙黏蛋白的调控因子，E-钙黏蛋白在细胞间连接中起关键作用，并受到两种转录因子ZEB1和ZEB2的抑制。在本研究中，通过实时定量PCR评估了30例非小细胞肺癌（NSCLC）中miR-200c、miR-9、ZEB-1、ZEB-2和E-钙黏蛋白的表达水平。与相邻正常组织相比，肿瘤组织中miR-9显著下调，而miR-200c的表达水平无显著变化。另一方面，ZEB1在mRNA水平上显著增加，ZEB2a减少。这些结果表明miR-9和ZEB1在肺癌发生中起作用，尽管临床病理相关性不明显。有必要进一步研究以评估其对肺癌治疗的意义。
