Biochemistry Department, Hospital General Universitario Santa Lucía , Cartagena , Spain.
Internal Medicine Department, Hospital General Universitario Santa Lucía , Cartagena , Spain.
Scand J Clin Lab Invest. 2019 Oct;79(6):455-458. doi: 10.1080/00365513.2019.1633686. Epub 2019 Jun 27.
Natriuretic peptides are a laboratory tool with significant implications for the diagnosis and prognosis of heart failure (HF). The International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) recommended that assays must be examined for sample stability because there appears to be assay dependent. We aimed to evaluate the stability of B-type natriuretic peptide (BNP) under different handling conditions and using a BNP assay from Fujirebio Diagnostics (Tokyo, Japan). BNP concentrations were measured in plasma EDTA samples from 11 subjects to evaluate the stability at room temperature and at 4 °C and in 10 subjects to check the stability of samples stored at -20 °C during 1 and 3 months. Stability limit was defined according to Spanish Society of Laboratory Medicine (SEQC-ML) recommendations. At room temperature and 4 °C, BNP concentrations decreased progressively in samples collected in both groups, remaining stable within four hours from collection. BNP concentrations also were stable within four hours from collection in whole blood at room temperature. Finally, at -20 °C, BNP concentrations remained stable in both groups at 1 and 3 months, respectively. According to our results, BNP, stored at room temperature or at 4 °C, should be assayed in the first four hours after collection. Besides, BNP was shown to be stable in whole blood for at least four hours at room temperature. If the testing cannot be performed within the first four hours, the plasma should be frozen and kept at -20 °C for up to 3 months.
利钠肽是一种实验室工具，对心力衰竭 (HF) 的诊断和预后具有重要意义。国际临床化学和实验室医学联合会 (IFCC) 建议，必须检查测定方法的样品稳定性，因为似乎存在依赖于测定方法的情况。我们旨在评估不同处理条件下和使用来自富士瑞生物诊断公司（日本东京）的 BNP 测定方法时 B 型利钠肽 (BNP) 的稳定性。在室温下和 4°C 下从 11 名受试者的 EDTA 血浆样本中测量 BNP 浓度，以评估样本在室温下和 4°C 下的稳定性，并在 10 名受试者中检查样本在 -20°C 下储存 1 和 3 个月的稳定性。稳定性极限是根据西班牙临床化学和实验室医学学会 (SEQC-ML) 的建议定义的。在室温下和 4°C 下，两组样本中 BNP 浓度逐渐降低，在收集后四小时内保持稳定。在室温下的全血中，BNP 浓度在收集后四小时内也保持稳定。最后，在 -20°C 下，BNP 浓度在两组中分别在 1 和 3 个月时保持稳定。根据我们的结果，BNP 在室温或 4°C 下储存时，应在收集后四小时内进行测定。此外，BNP 在室温下的全血中至少可稳定四小时。如果在最初四小时内无法进行测试，则应将血浆冷冻并保持在 -20°C 下最长 3 个月。
