用于测定人血浆中他莫昔芬及其代谢物的新型超高效液相色谱-串联质谱法，在患者中的应用。
New UPLC-MS/MS assay for the determination of tamoxifen and its metabolites in human plasma, application to patients.
作者信息
Bobin-Dubigeon Christine, Campone Mario, Rossignol Elsa, Salaun Estelle, Amiand Marie-Bernadette, Bard Jean-Marie
机构信息
Biopathology Department ICO René Gauducheau, Bd J Monod, 44805 Nantes Saint Herblain Cedex, France.
Faculté de Pharmacie, Université de Nantes, EA 2160 MMS, IUML FR3473 CNRS, 1 rue Gaston Veil, 44000 Nantes, France.
出版信息
Future Sci OA. 2019 Mar 22;5(5):FSO374. doi: 10.2144/fsoa-2018-0113. eCollection 2019 Jun.
AIM
A rapid UPLC-MS/MS method for the determination of tamoxifen (TAM), -desmethyltamoxifen, 4-hydroxytamoxifen and endoxifen in human plasma was validated, after a simple protein precipitation.
MATERIALS AND METHODS
The analysis was achieved on a C analytical column, using a gradient elution with a mobile phase of water and acetonitrile for 4.5 min.
RESULTS
The validated method demonstrated good linearity between 1 and 500 ng/ml for TAM and -desmethyltamoxifen; between 0.2 and 100 ng/ml for endoxifen and between 0.1 and 50 ng/ml for 4-hydroxytamoxifen. The method also provided satisfactory results in terms of within day and between day imprecisions and accuracy, and also in terms of time stability and specificity.
CONCLUSION
The method is applied routinely for TAM monitoring from patients undergoing therapy.
目的
建立一种快速的超高效液相色谱-串联质谱法（UPLC-MS/MS），用于在简单的蛋白质沉淀后测定人血浆中的他莫昔芬（TAM）、去甲基他莫昔芬、4-羟基他莫昔芬和内昔芬。
材料与方法
分析在C分析柱上进行，使用水和乙腈作为流动相进行梯度洗脱4.5分钟。
结果
验证后的方法显示，TAM和去甲基他莫昔芬在1至500 ng/ml之间具有良好的线性；内昔芬在0.2至100 ng/ml之间，4-羟基他莫昔芬在0.1至50 ng/ml之间具有良好的线性。该方法在日内和日间精密度与准确度方面，以及时间稳定性和特异性方面也提供了令人满意的结果。
结论
该方法常规应用于接受治疗患者的TAM监测。
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