自组装的 Au@Fe 核/卫星型磁性纳米粒子用于多功能生物分子功能化。
Self-Assembled Au@Fe Core/Satellite Magnetic Nanoparticles for Versatile Biomolecule Functionalization.
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy , University of Michigan , Ann Arbor , Michigan 48109 , United States.
IMRA America, Inc. , 1044 Woodridge Avenue , Ann Arbor , Michigan 48105 , United States.
出版信息
ACS Appl Mater Interfaces. 2019 Jul 10;11(27):23858-23869. doi: 10.1021/acsami.9b05544. Epub 2019 Jun 27.
Although the functionalization of magnetic nanoparticles (MNPs) with biomolecules has been widely explored for various biological applications, achieving efficient bioconjugations with a wide range of biomolecules through a single, universal, and versatile platform remains a challenge, which may significantly impact their applications' outcomes. Here, we report a novel MNP platform composed of Au@Fe core/satellite nanoparticles (CSNPs) for versatile and efficient bioconjugations. The engineering of the CSNPs is facilely formed through the self-assembly of ultrasmall gold nanoparticles (AuNPs, 2-3 nm in diameter) around MNPs with a polysiloxane-containing polymer coating. The formation of the hybrid magnetic nanostructure is revealed by absorption spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), element analysis using atomic absorption spectroscopy, and vibrating sample magnetometer. The versatility of biomolecule loading to the CSNP is revealed through the bioconjugation of a wide range of relevant biomolecules, including streptavidin, antibodies, peptides, and oligonucleotides. Characterizations including DLS, TEM, lateral flow strip assay, fluorescence assay, giant magnetoresistive nanosensor array, high-performance liquid chromatography, and absorption spectrum are performed to further confirm the efficiency of various bioconjugations to the CSNP. In conclusion, this study demonstrates that the CSNP is a novel MNP-based platform that offers versatile and efficient surface functionalization with various biomolecules.
虽然将磁性纳米粒子 (MNPs) 与生物分子进行功能化已经在各种生物应用中得到了广泛的探索,但通过单个通用且多功能的平台实现与广泛的生物分子的高效生物偶联仍然是一个挑战,这可能会显著影响它们的应用结果。在这里,我们报告了一种由 Au@Fe 核/卫星纳米粒子 (CSNPs) 组成的新型 MNP 平台,用于多功能和高效的生物偶联。CSNPs 的工程设计通过将含有聚硅氧烷的聚合物涂层周围的 MNPs 周围的超小金纳米粒子 (AuNPs,直径 2-3nm) 自组装来轻松形成。通过吸收光谱、动态光散射 (DLS)、透射电子显微镜 (TEM)、原子吸收光谱法进行的元素分析以及振动样品磁强计揭示了混合磁性纳米结构的形成。通过将各种相关生物分子(包括链霉亲和素、抗体、肽和寡核苷酸)与 CSNP 的生物偶联,揭示了生物分子加载到 CSNP 的多功能性。包括 DLS、TEM、侧流条检测、荧光检测、巨磁电阻纳米传感器阵列、高效液相色谱和吸收光谱在内的表征进一步证实了各种生物偶联物与 CSNP 的效率。总之,这项研究表明 CSNP 是一种新型的基于 MNP 的平台,可提供多功能和高效的表面功能化,用于各种生物分子。