Departamento de Quíımica Inorgánica, Facultad de Ciencia y Tecnología, UPV/EHU, Apdo. 644, 48080 Bilbao, Spain.
Nanotechnology. 2012 Aug 10;23(31):315102. doi: 10.1088/0957-4484/23/31/315102.
The interaction of nanoparticles with cells has been a focus of interest during the past decade. We report the fabrication and characterization of hydrosoluble Fe₃O₄@Au nanoparticles functionalized with biocompatible and fluorescent molecules and their interaction with cell cultures by visualizing them with confocal microscopy. Gold covered iron oxide nanoparticles were synthesized by reducing metal salts in the presence of oleylamine and oleic acid. The functionalization of these particles with an amphiphilic polymer provides a water soluble corona as well as the possibility to incorporate different molecules relevant for bio-applications such as poly(ethylene glycol), glucose or a cadaverine derived dye. The particle size, and the presence of polymer layers and conjugated molecules were characterized and confirmed by transmission electron microscopy, thermogravimetric measurements and infrared spectroscopy. A complete magnetic study was performed, showing that gold provides an optimum coating, which enhances the superparamagnetic behaviour observed above 10-15 K in this kind of nanoparticle. The interaction with cells and the cytotoxicity of the Fe₃O₄@Au preparations were determined upon incubation with the HeLa cell line. These nanoparticles showed no cytotoxicity when evaluated by the MTT assay and it was demonstrated that nanoparticles clearly interacted with the cells, showing a higher level of accumulation in the cells for glucose conjugated nanoparticles.
在过去的十年中，纳米粒子与细胞的相互作用一直是研究的热点。我们报告了通过共焦显微镜可视化来制备和表征具有生物相容性和荧光分子的可水溶的 Fe₃O₄@Au 纳米粒子，并研究它们与细胞培养物的相互作用。金覆盖的氧化铁纳米粒子是通过在油胺和油酸的存在下还原金属盐合成的。这些粒子的功能化与两亲聚合物提供了水溶性冠，以及有可能掺入与生物应用相关的不同分子，如聚（乙二醇）、葡萄糖或来源于尸胺的染料。通过透射电子显微镜、热重测量和红外光谱对粒径以及聚合物层和共轭分子的存在进行了表征和确认。进行了完整的磁性研究，表明金提供了最佳的涂层，增强了在这种纳米粒子中高于 10-15 K 观察到的超顺磁行为。通过与 HeLa 细胞系孵育，确定了 Fe₃O₄@Au 制剂与细胞的相互作用和细胞毒性。通过 MTT 测定评估时，这些纳米粒子没有显示出细胞毒性，并且证明纳米粒子与细胞明显相互作用，对于葡萄糖共轭的纳米粒子，在细胞中的积累水平更高。
