Shepard Kayla N, Haffner John C, Neal Dwana L, Grubbs Steven T, Pearce Greg L
Boehringer Ingelheim Vetmedica, St. Joseph, MO (Shepard, Neal, Grubbs).
Middle Tennessee State University, Murfreesboro, TN (Haffner).
J Vet Diagn Invest. 2019 Jul;31(4):585-587. doi: 10.1177/1040638719860877. Epub 2019 Jun 27.
Plasma adrenocorticotropic hormone (ACTH) concentration is used in the diagnosis of pituitary pars intermedia dysfunction (PPID) in horses. We enrolled 10 horses, 5 PPID-positive and 5 PPID-negative, in our study, September 20-22, 2016. On day 0, 5 mL of whole blood was collected into each of 6 EDTA tubes and immediately placed in a refrigerator at 7°C. One tube was centrifuged within 15 min of collection, followed by centrifugation of one tube from each horse at 4, 8, 12, 24, and 36 h following collection. At each time, centrifuged plasma was pipetted into 1.5-mL polypropylene tubes and stored at -80°C. None of the plasma samples were turbid, hemolyzed, or icteric. Plasma was shipped frozen with cold packs overnight to the Animal Health Diagnostic Center of Cornell University (Ithaca, NY) for analysis. The percent change from baseline (PCFB) was reported to standardize the data given that baseline values differed. The mean PCFB was 2.8 (95% confidence interval: -2.9%, 7.0%). Neither refrigeration of whole blood for up to 36 h prior to centrifugation nor freezing affected plasma ACTH concentrations significantly.
血浆促肾上腺皮质激素（ACTH）浓度用于诊断马的垂体中间部功能障碍（PPID）。在2016年9月20日至22日，我们招募了10匹马进行研究，其中5匹为PPID阳性，5匹为PPID阴性。在第0天，将5毫升全血分别采集到6个乙二胺四乙酸（EDTA）管中，并立即置于7°C的冰箱中。在采集后15分钟内对其中一管进行离心，随后在采集后的4、8、12、24和36小时对每匹马的一管进行离心。每次离心后，将血浆吸移至1.5毫升聚丙烯管中，并储存在-80°C。所有血浆样本均未出现浑浊、溶血或黄疸。血浆用冰袋冷冻运输过夜，送至康奈尔大学动物健康诊断中心（纽约州伊萨卡）进行分析。鉴于基线值不同，报告了相对于基线的变化百分比（PCFB）以标准化数据。平均PCFB为2.8（95%置信区间：-2.9%，7.0%）。在离心前将全血冷藏长达36小时以及冷冻均未显著影响血浆ACTH浓度。
