腺苷的放射酶法测定

Radioenzymatic determination of adenosine.

作者信息

Camici M, Mura U, Del Corso A, Gini S, Ipata P L

机构信息

Dipartimento di Fisiologia e Biochimica, Universitá di Pisa, Italy.

出版信息

Anal Biochem. 1987 Nov 1;166(2):253-6. doi: 10.1016/0003-2697(87)90572-0.

DOI:10.1016/0003-2697(87)90572-0

PMID:3124668

Abstract

Adenosine has been measured at the nanomolar level by an enzymatic radioactive assay. The nucleoside is converted into [U-14C]ribose-labeled inosine via the following reactions: adenosine + H2O----adenine + ribose (adenosine nucleosidase); adenine + [U-14C]ribose 1-phosphate in equilibrium with T[U-14C]ribose-adenosine + Pi (adenosine phosphorylase); [U-14C]ribose-adenosine + H2O----[U-14C]ribose-inosine + NH3 (adenosine deaminase). The radioactivity of inosine, separated by thin-layer chromatography, is a measure of the adenosine initially present.

摘要

腺苷已通过酶促放射性测定法在纳摩尔水平进行了测量。该核苷通过以下反应转化为[U-14C]核糖标记的肌苷：腺苷+H2O→腺嘌呤+核糖（腺苷核苷酶）；腺嘌呤+[U-14C]核糖1-磷酸与T[U-14C]核糖-腺苷+Pi处于平衡状态（腺苷磷酸化酶）；[U-14C]核糖-腺苷+H2O→[U-14C]核糖-肌苷+NH3（腺苷脱氨酶）。通过薄层色谱法分离的肌苷的放射性是最初存在的腺苷的量度。