GD Animal Health, Deventer, The Netherlands.
PLoS One. 2019 Jun 27;14(6):e0218481. doi: 10.1371/journal.pone.0218481. eCollection 2019.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of a devastating pig disease present all over the world. The remarkable genetic variation of PRRSV, makes epidemiological and molecular analysis of circulating viruses highly important to review current diagnostic tools and vaccine efficacy. Monitoring PRRS viruses supports modern herd management by explaining the source of found viruses, either internally or externally from the herd. No epidemiological or molecular study has been published on circulating PRRS-viruses in the Netherlands, since the early nineties. Therefore, the objective of this study is to investigate circulating PRRS-viruses in the Netherlands in 2014, 2015 and 2016 on a molecular level by sequencing ORF2, ORF3, ORF4, ORF5, ORF6 and ORF7. The results demonstrate that the 74 PRRSV strains belong to PRRSV-1, but the diversity among strains is high, based on nucleotide identity, individual ORF length and phylogenetic trees of individual ORFs. Furthermore, the data presented here show that the phylogenetic topology of some viruses is ORF dependent and suggests recombination. The identity of the strain of interest might be misinterpreted and wrong conclusions may be drawn in a diagnostic and epidemiological perspective, when only ORF5 is analyzed, as performed in many routine sequencing procedures.
猪繁殖与呼吸综合征病毒（PRRSV）是一种具有毁灭性的猪病的病原体，在全球范围内都有出现。PRRSV 的显著遗传变异使得对流行病毒进行流行病学和分子分析对于评估当前诊断工具和疫苗效力非常重要。监测 PRRS 病毒有助于通过解释在群内或群外发现的病毒来源来支持现代养殖管理。自 90 年代初以来，荷兰就没有关于循环 PRRS 病毒的流行病学或分子研究。因此，本研究的目的是通过测序 ORF2、ORF3、ORF4、ORF5、ORF6 和 ORF7，从分子水平上调查 2014 年、2015 年和 2016 年荷兰循环 PRRSV 的情况。结果表明，74 株 PRRSV 株属于 PRRSV-1，但基于核苷酸同一性、单个 ORF 长度和单个 ORF 的系统发育树，株间多样性很高。此外，这里提供的数据表明，一些病毒的系统发育拓扑结构是依赖于 ORF 的，并提示存在重组。如果只分析 ORF5，就像许多常规测序程序那样，可能会误解感兴趣的病毒株的身份，并在诊断和流行病学方面得出错误的结论。
