纳米零价铁对牛粪厌氧消化过程中抗生素抗性基因的影响。
Effects of nano-zerovalent iron on antibiotic resistance genes during the anaerobic digestion of cattle manure.
机构信息
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Research Center of Recycle Agricultural Engineering and Technology of Shaanxi Province, Northwest A&F University, Yangling, Shaanxi 712100, China.
出版信息
Bioresour Technol. 2019 Oct;289:121688. doi: 10.1016/j.biortech.2019.121688. Epub 2019 Jun 22.
This study investigated the effects of adding nano-zerovalent iron (nZVI) at three concentrations (0, 80, and 160 mg/L) on the methane yield and the fate of antibiotic resistance genes (ARGs) during the anaerobic digestion (AD) of cattle manure. The addition of nZVI effectively enhanced the methane yield, where it significantly increased by 6.56% with 80 mg/L nZVI and by 6.43% with 160 mg/L nZVI. The reductions in the abundances of ARGs and Tn916/1545 were accelerated by adding 160 mg/L nZVI after AD. Microbial community analysis showed that nZVI mainly increased the abundances of bacteria with roles in hydrolysis and acidogenesis, whereas it reduced the abundance of Acinetobacter. Redundancy analysis indicated that the changes in mobile genetic elements made the greatest contribution to the fate of ARGs. The results suggest that 160 mg/L nZVI is a suitable additive for reducing the risks due to ARGs in AD.
本研究考察了在三个浓度（0、80 和 160mg/L）下添加纳米零价铁（nZVI）对牛粪厌氧消化（AD）过程中甲烷产量和抗生素抗性基因（ARGs）归宿的影响。添加 nZVI 可有效提高甲烷产量，添加 80mg/L nZVI 可使甲烷产量显著增加 6.56%，添加 160mg/L nZVI 可使甲烷产量增加 6.43%。添加 160mg/L nZVI 可加速 AD 后 ARGs 和 Tn916/1545 的丰度降低。微生物群落分析表明，nZVI 主要增加了在水解和产酸阶段起作用的细菌的丰度，而降低了不动杆菌的丰度。冗余分析表明，移动遗传元件的变化对 ARGs 的归宿贡献最大。结果表明，160mg/L nZVI 是一种适合添加的物质，可降低 AD 中 ARGs 带来的风险。
Zotero 插件