Kim S J
Membrane Research Institute, University City Science Center, Philadelphia, PA 19104.
Biochem Biophys Res Commun. 1988 Jan 29;150(2):870-6. doi: 10.1016/0006-291x(88)90473-1.
Rat alveolar macrophages incubated with (15S), 15-hydroperoxy-5,8, 11-cis-13-trans-eicosatetraenoic acid (15-HPETE) in the presence of the calcium ionophore A23187 produced a series of polar compounds. Reverse-phase high performance liquid chromatography (RP-HPLC) was used to purify these compounds and their identities were characterized by physical criteria (UV spectroscopy, GC/MS) and by co-elution with synthetic compounds. Alveolar macrophage-derived materials proved to be lipoxin A4 [LXA4; (5S, 6R, 15S)-5,6,15-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid], lipoxin B4[LXB4; (5S, 14R, 15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis- eicosatetraenoic acid], and a structural isomer. These results indicate that rat alveolar macrophages have the capacity to generate lipoxins and related compounds.
在钙离子载体A23187存在的情况下，用（15S）-15-氢过氧-5,8,11-顺式-13-反式-二十碳四烯酸（15-HPETE）孵育大鼠肺泡巨噬细胞，产生了一系列极性化合物。采用反相高效液相色谱（RP-HPLC）对这些化合物进行纯化，并通过物理标准（紫外光谱、气相色谱/质谱）以及与合成化合物共洗脱来表征它们的身份。肺泡巨噬细胞衍生的物质被证明是脂氧素A4 [LXA4；（5S，6R，15S）-5,6,15-三羟基-7,9,13-反式-11-顺式-二十碳四烯酸]、脂氧素B4 [LXB4；（5S，14R，15S）-5,14,15-三羟基-6,10,12-反式-8-顺式-二十碳四烯酸]以及一种结构异构体。这些结果表明，大鼠肺泡巨噬细胞具有生成脂氧素及相关化合物的能力。
