在人血小板-粒细胞混合悬液中内源性花生四烯酸向脂氧素的跨细胞转化。
Transcellular conversion of endogenous arachidonic acid to lipoxins in mixed human platelet-granulocyte suspensions.
作者信息
Edenius C, Haeggström J, Lindgren J A
机构信息
Department of Physiological Chemistry, Karolinska Institutet, Stockholm, Sweden.
出版信息
Biochem Biophys Res Commun. 1988 Dec 15;157(2):801-7. doi: 10.1016/s0006-291x(88)80320-6.
Incubation of mixed human platelet/granulocyte suspensions with ionophore A23187 led to a platelet dependent formation of several lipoxin isomers from endogenous substrate. The major metabolite coeluted with authentic lipoxin A4 (5(S), 6(R), 15(S)-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid) in several HPLC-systems and showed an identical UV-spectrum. Furthermore, a similar profile of lipoxins was formed in pure platelet suspensions incubated with exogenous leukotriene A4 (5(S) -5, 6-oxido-7,9-trans-11,14-cis-eicosatetraenoic acid). The conversion of exogenous leukotriene A4 to lipoxin A4 was markedly increased in the presence of ionophore A23187.
将人血小板/粒细胞混合悬浮液与离子载体A23187一起孵育，可导致从内源性底物中以血小板依赖的方式形成几种脂氧素异构体。在几种高效液相色谱系统中，主要代谢产物与 authentic脂氧素A4（5(S), 6(R), 15(S)-三羟基-7,9,13-反式-11-顺式-二十碳四烯酸）共洗脱，并显示出相同的紫外光谱。此外，在用外源性白三烯A4（5(S)-5, 6-环氧-7,9-反式-11,14-顺式-二十碳四烯酸）孵育的纯血小板悬浮液中形成了类似的脂氧素谱。在离子载体A23187存在下，外源性白三烯A4向脂氧素A4的转化明显增加。
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