Nakajo S, Nakaya K, Nakamura Y
Laboratory of Biological Chemistry, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Biochem Int. 1987 Aug;15(2):321-7.
Filamin and vinculin from chicken gizzards were significantly phosphorylated in vitro by casein kinases 1 and 2, but not by alpha-actinin. Antisera raised against these actin-binding proteins immunoprecipitated the phosphorylated proteins corresponding to filamin and vinculin, but no phosphoprotein corresponding to alpha-actinin was detected. These results suggest that filamin and vinculin are phosphorylated in vivo but alpha-actinin is not.
鸡砂囊中的细丝蛋白和纽蛋白在体外被酪蛋白激酶1和2显著磷酸化，但未被α-辅肌动蛋白磷酸化。针对这些肌动蛋白结合蛋白产生的抗血清免疫沉淀出了与细丝蛋白和纽蛋白相对应的磷酸化蛋白，但未检测到与α-辅肌动蛋白相对应的磷酸蛋白。这些结果表明，细丝蛋白和纽蛋白在体内被磷酸化，而α-辅肌动蛋白则未被磷酸化。
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