超声诱导兔血脊髓屏障开放。
Ultrasound-Induced Blood-Spinal Cord Barrier Opening in Rabbits.
机构信息
Sorbonne Universite, Neurosurgery Department, AP-HP, Hopital de la Pitie Salpêtrière, Paris, France; Advanced Surgical Research Technology Laboratory, Sorbonne Universite, Paris, France.
Sorbonne Universite, Department of Neuropathology, AP-HP, Hopital de la Pitie Salpêtrière, Paris, France.
出版信息
Ultrasound Med Biol. 2019 Sep;45(9):2417-2426. doi: 10.1016/j.ultrasmedbio.2019.05.022. Epub 2019 Jun 24.
The blood-spinal cord barrier (BSCB) considerably limits the delivery and efficacy of treatments for spinal cord diseases. The blood-brain barrier can be safely opened with low-intensity pulsed ultrasound when microbubbles are simultaneously administered intravenously. This technique was tested on the BSCB in a rabbit model in this work. Twenty-three segments of spinal cord were sonicated with a 1-MHz unfocused pulsed ultrasound device and compared with non-sonicated segments. BSCB disruption was assessed using Evan's blue dye (EBD) extravasation. Tolerance was assessed by histologic analysis. An increased EBD concentration indicating BSCB disruption was clearly observed in sonicated segments compared with controls (p = 0.004). On one animal, which received 10 sonications, repetitive BSCB disruptions revealed no evidence of cumulative toxicity. BSCB can be disrupted using an unfocused pulsed ultrasound device in combination with microbubbles without neurotoxicity even in case of repeated sonications.
血脊髓屏障（BSCB）极大地限制了脊髓疾病治疗方法的递送和效果。当同时静脉内给予微泡时，可以安全地用低强度脉冲超声打开血脑屏障。这项技术在本工作中在兔模型中对 BSCB 进行了测试。用 1MHz 非聚焦脉冲超声设备对 23 个脊髓段进行了超声处理，并与未超声处理的段进行了比较。使用伊文思蓝染料（EBD）渗出来评估 BSCB 破坏。通过组织学分析评估耐受性。与对照组相比，在超声处理的段中明显观察到 EBD 浓度增加，表明 BSCB 破坏（p=0.004）。在一只接受 10 次超声处理的动物中，重复 BSCB 破坏没有显示出累积毒性的证据。即使在重复超声处理的情况下，也可以使用非聚焦脉冲超声设备与微泡联合破坏 BSCB，而没有神经毒性。
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