Barnett Institute of Chemical and Biological Analysis and Departments of Chemistry & Chemical Biology and Pharmaceutical Sciences, Northeastern University, Boston, MA, USA.
Departments of Chemistry and Molecular Biosciences and the Proteomics Center of Excellence, Northwestern University, Evanston, IL, USA.
Nat Methods. 2019 Jul;16(7):587-594. doi: 10.1038/s41592-019-0457-0. Epub 2019 Jun 27.
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.
一个基因可以产生许多功能不同的蛋白异构体，每种异构体都具有独特的分子质量。自上而下的质谱分析可用于分析完整蛋白质和蛋白异构体。在此，自上而下蛋白质组学联盟的成员提供了一个决策树，指导研究人员使用稳健的协议，对来自不同复杂程度混合物的完整蛋白质（抗体、膜蛋白等）进行质谱分析。我们还使用蛋白质标准样品提供了跨平台分析基准，以便用户评估自己的水平。
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