通过自上而下的质谱法破译组合翻译后修饰。
Deciphering combinatorial post-translational modifications by top-down mass spectrometry.
机构信息
Department of Chemistry, University of Texas at Austin, Austin, TX 78712, USA.
出版信息
Curr Opin Chem Biol. 2022 Oct;70:102180. doi: 10.1016/j.cbpa.2022.102180. Epub 2022 Jun 29.
Abstract
Post-translational modifications (PTMs) create vast structural and functional diversity of proteins, ultimately modulating protein function and degradation, influencing cellular signaling, and regulating transcription. The combinatorial patterns of PTMs increase the heterogeneity of proteins and further mediates their interactions. Advances in mass spectrometry-based proteomics have resulted in identification of thousands of proteins and allowed characterization of numerous types and sites of PTMs. Examination of intact proteins, termed the top-down approach, offers the potential to map protein sequences and localize multiple PTMs on each protein, providing the most comprehensive cataloging of proteoforms. This review describes some of the dividends of using mass spectrometry to analyze intact proteins and showcases innovative strategies that have enhanced the promise of top-down proteomics for exploring the impact of combinatorial PTMs in unsurpassed detail.
摘要
翻译后的内容为
翻译后内容
相似文献
1
Deciphering combinatorial post-translational modifications by top-down mass spectrometry.通过自上而下的质谱法破译组合翻译后修饰。
Curr Opin Chem Biol. 2022 Oct;70:102180. doi: 10.1016/j.cbpa.2022.102180. Epub 2022 Jun 29.
2
Characterization of Proteoforms with Unknown Post-translational Modifications Using the MIScore.使用MIScore对具有未知翻译后修饰的蛋白质异构体进行表征。
J Proteome Res. 2016 Aug 5;15(8):2422-32. doi: 10.1021/acs.jproteome.5b01098. Epub 2016 Jul 1.
3
Top-down mass spectrometry for the analysis of combinatorial post-translational modifications.自上而下的质谱法用于分析组合性翻译后修饰。
Mass Spectrom Rev. 2013 Jan-Feb;32(1):27-42. doi: 10.1002/mas.21348. Epub 2012 Jun 20.
4
Top-down characterization of chicken core histones.鸡核心组蛋白的自上而下的表征。
J Proteomics. 2018 Jul 30;184:34-38. doi: 10.1016/j.jprot.2018.06.007. Epub 2018 Jun 20.
5
Characterization of Proteoform Post-Translational Modifications by Top-Down and Bottom-Up Mass Spectrometry in Conjunction with Annotations.通过与注释相结合的自上而下和自下而上的质谱法对蛋白质翻译后修饰进行特征描述。
J Proteome Res. 2023 Oct 6;22(10):3178-3189. doi: 10.1021/acs.jproteome.3c00207. Epub 2023 Sep 20.
6
Top-Down Proteomics and the Challenges of True Proteoform Characterization.自上而下的蛋白质组学和真正的蛋白质组特征分析面临的挑战。
J Proteome Res. 2023 Dec 1;22(12):3663-3675. doi: 10.1021/acs.jproteome.3c00416. Epub 2023 Nov 8.
7
Improving Proteoform Identifications in Complex Systems Through Integration of Bottom-Up and Top-Down Data.通过整合自下而上和自上而下的数据改进复杂系统中的蛋白质异构体鉴定
J Proteome Res. 2020 Aug 7;19(8):3510-3517. doi: 10.1021/acs.jproteome.0c00332. Epub 2020 Jul 10.
8
Constructing Human Proteoform Families Using Intact-Mass and Top-Down Proteomics with a Multi-Protease Global Post-Translational Modification Discovery Database.使用完整质量和自上而下的蛋白质组学以及具有多种蛋白酶的全局翻译后修饰发现数据库构建人类蛋白质形式家族。
J Proteome Res. 2019 Oct 4;18(10):3671-3680. doi: 10.1021/acs.jproteome.9b00339. Epub 2019 Sep 18.
9
Analysis of cardiac troponin proteoforms by top-down mass spectrometry.采用自上而下质谱法分析心肌肌钙蛋白蛋白异构体
Methods Enzymol. 2019;626:347-374. doi: 10.1016/bs.mie.2019.07.029. Epub 2019 Aug 27.
10
Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications.质谱为主的自上而下蛋白质组学：技术进展及生物医学应用的更新。
Anal Methods. 2024 Jul 18;16(28):4664-4682. doi: 10.1039/d4ay00651h.
引用本文的文献
1
The role of multi-omics in biomarker discovery, diagnosis, prognosis, and therapeutic monitoring of tissue repair and regeneration processes.多组学在组织修复和再生过程的生物标志物发现、诊断、预后及治疗监测中的作用。
J Orthop Translat. 2025 Aug 8;54:131-151. doi: 10.1016/j.jot.2025.07.006. eCollection 2025 Sep.
2
Structural Heterogeneity of Proteoform-Ligand Complexes in Adenosine Monophosphate-Activated Protein Kinase Uncovered by Integrated Top-Down Mass Spectrometry.通过整合自上而下质谱法揭示的单磷酸腺苷激活蛋白激酶中蛋白变体-配体复合物的结构异质性
J Am Chem Soc. 2025 Aug 14. doi: 10.1021/jacs.5c06950.
3
Monitoring Functional Posttranslational Modifications Using a Data-Driven Proteome Informatic Pipeline.使用数据驱动的蛋白质组信息学流程监测功能性翻译后修饰
Proteomics. 2025 Apr;25(8):e202400238. doi: 10.1002/pmic.202400238. Epub 2025 Mar 18.
4
How to Deal With Internal Fragment Ions?如何处理内部碎片离子？
Mol Cell Proteomics. 2025 May;24(5):100896. doi: 10.1016/j.mcpro.2024.100896. Epub 2025 Feb 13.
5
Deciphering Proteoform Landscape of Mammary Carcinoma by Top-Down Proteomics.通过自上而下蛋白质组学解析乳腺癌的蛋白质异构体图谱
J Proteome Res. 2025 Mar 7;24(3):1425-1438. doi: 10.1021/acs.jproteome.4c01044. Epub 2025 Feb 12.
6
A Conformation-Specific Approach to Native Top-down Mass Spectrometry.一种针对天然自上而下质谱分析的构象特异性方法。
J Am Soc Mass Spectrom. 2024 Dec 4;35(12):3203-3213. doi: 10.1021/jasms.4c00361. Epub 2024 Oct 25.
7
Comprehensive Overview of Bottom-Up Proteomics Using Mass Spectrometry.基于质谱的自下而上蛋白质组学综合概述
ACS Meas Sci Au. 2024 Jun 4;4(4):338-417. doi: 10.1021/acsmeasuresciau.3c00068. eCollection 2024 Aug 21.
8
Dissecting the functional behavior of the differentially phosphorylated prolyl isomerase, Pin1.解析差异磷酸化脯氨酰异构酶 Pin1 的功能行为。
Protein Sci. 2024 Sep;33(9):e5138. doi: 10.1002/pro.5138.
9
Top-down proteomics.自上而下蛋白质组学
Nat Rev Methods Primers. 2024;4(1). doi: 10.1038/s43586-024-00318-2. Epub 2024 Jun 13.
10
Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications.质谱为主的自上而下蛋白质组学：技术进展及生物医学应用的更新。
Anal Methods. 2024 Jul 18;16(28):4664-4682. doi: 10.1039/d4ay00651h.
本文引用的文献
1
Deciphering protein post-translational modifications using chemical biology tools.使用化学生物学工具解析蛋白质翻译后修饰
Nat Rev Chem. 2020 Dec;4(12):674-695. doi: 10.1038/s41570-020-00223-8. Epub 2020 Oct 6.
2
Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry.利用电子捕获解离和循环离子淌度谱技术增强自上而下的蛋白质鉴定。
Anal Chem. 2022 Mar 8;94(9):3888-3896. doi: 10.1021/acs.analchem.1c04870. Epub 2022 Feb 21.
3
The Blood Proteoform Atlas: A reference map of proteoforms in human hematopoietic cells.血液蛋白质组图谱：人类造血细胞中蛋白质组的参考图谱。
Science. 2022 Jan 28;375(6579):411-418. doi: 10.1126/science.aaz5284. Epub 2022 Jan 27.
4
Characterization of large intact protein ions by mass spectrometry: What directions should we follow?通过质谱法对完整大蛋白离子进行表征：我们应遵循哪些方向？
Biochim Biophys Acta Proteins Proteom. 2022 Apr 1;1870(4):140758. doi: 10.1016/j.bbapap.2022.140758. Epub 2022 Jan 22.
5
Towards understanding the formation of internal fragments generated by collisionally activated dissociation for top-down mass spectrometry.为了理解碰撞激活解离产生的自上而下质谱内片段的形成。
Anal Chim Acta. 2022 Feb 15;1194:339400. doi: 10.1016/j.aca.2021.339400. Epub 2021 Dec 30.
6
Native Mass Spectrometry and Surface Induced Dissociation Provide Insight into the Post-Translational Modifications of Tetrameric AQP0 Isolated from Bovine Eye Lens.天然质谱法和表面诱导解吸技术揭示了从牛眼晶状体中分离的四聚体 AQP0 的翻译后修饰。
Anal Chem. 2022 Jan 25;94(3):1515-1519. doi: 10.1021/acs.analchem.1c04322. Epub 2022 Jan 11.
7
Extending Native Top-Down Electron Capture Dissociation to MDa Immunoglobulin Complexes Provides Useful Sequence Tags Covering Their Critical Variable Complementarity-Determining Regions.将原生自上而下的电子捕获解离扩展至兆道尔顿免疫球蛋白复合物，可提供覆盖其关键可变互补决定区的有用序列标签。
Anal Chem. 2021 Dec 7;93(48):16068-16075. doi: 10.1021/acs.analchem.1c03740. Epub 2021 Nov 23.
8
Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation.通过电子转移解离过程中的离子并行泊车提高完整蛋白质的序列分析。
Anal Chem. 2021 Nov 30;93(47):15728-15735. doi: 10.1021/acs.analchem.1c03652. Epub 2021 Nov 17.
9
Surface-induced Dissociation Mass Spectrometry as a Structural Biology Tool.表面诱导解吸质谱法作为结构生物学工具。
Chem Rev. 2022 Apr 27;122(8):7442-7487. doi: 10.1021/acs.chemrev.1c00309. Epub 2021 Nov 2.
10
Spectrum of Apolipoprotein AI and Apolipoprotein AII Proteoforms and Their Associations With Indices of Cardiometabolic Health: The CARDIA Study.载脂蛋白 AI 和载脂蛋白 AII 蛋白亚型谱及其与心脏代谢健康指标的关系：CARDIA 研究。
J Am Heart Assoc. 2021 Sep 7;10(17):e019890. doi: 10.1161/JAHA.120.019890. Epub 2021 Sep 2.
Zotero 插件