Li Wen Jie, Yang Huan Min, Lian Shuai, Xu Bin, Wang Li Peng, Zang Shu Cheng, Yuan Jian Bin, Ding Meng Yuan
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2019 Feb;35(2):135-139. doi: 10.12047/j.cjap.5722.2019.030.
To study the effects of prenatal cold stress on the behavior and mood of offspring in pregnant rats.
Six SPF-class Wister pregnant rats were randomly divided into normal temperature control group and cold stress group with 3 rats in each group. The pregnant female rats in the normal temperature control group were kept in the environment of (22 ±2)℃, and the pregnant female rats in the cold stress group were placed in the artificial intelligence climate chamber at(4 ±0.1)℃ for 7 days before the birth, and the young rats were divided into normal temperature after the young rats were born. After the young rats were born, they were divided into normal temperature control group of male rats (MR, 22), normal temperature control group of mother rats (FR, 15), cold stress group of male rats (MC, 15), and cold stress group of female rats (FC, 15) .In the fourth generation of the offspring, the open field experiment and the elevated cross maze test were carried out.
In the open field experiment, there was no significant difference in spontaneous activity and exploration behavior between the normal temperature control group and the cold stress group (P＞0.05). In the elevated plus maze experiment, the retention time of the open arms, the number of open arms and the distance of the male and female rats in the cold stress group were significantly higher than those in the normal temperature control group (P＜0.05).
Prenatal maternal cold stress has no significant effect on spontaneous activity, exploration behavior and activity level of offspring, but the offspring have obvious abnormal behaviors with reduced anxiety behavior.
研究孕期冷应激对孕鼠后代行为和情绪的影响。
将6只SPF级Wister孕鼠随机分为常温对照组和冷应激组，每组3只。常温对照组的孕鼠饲养于（22±2）℃环境中，冷应激组的孕鼠在分娩前7天置于（4±0.1）℃的人工智能气候箱中，幼鼠出生后分为常温组。幼鼠出生后，分为雄性常温对照组（MR，22只）、雌性常温对照组（FR，15只）、雄性冷应激组（MC，15只）和雌性冷应激组（FC，15只）。在后代的第四代进行旷场实验和高架十字迷宫试验。
旷场实验中，常温对照组与冷应激组的自发活动和探究行为无显著差异（P＞0.05）。在高架十字迷宫实验中，冷应激组雌雄大鼠的开放臂停留时间、开放臂次数和移动距离均显著高于常温对照组（P＜0.05）。
孕期母体冷应激对后代的自发活动、探究行为和活动水平无显著影响，但后代出现明显的异常行为，焦虑行为减少。
