Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Tianma Japan, Ltd., Shin-Kawasaki Mitsui Building West Tower 28F 1-1-2, Kashimada, Saiwai-ku, Kawasaki, Kanagawa 212-0058, Japan.
Lab Chip. 2019 Aug 7;19(15):2581-2588. doi: 10.1039/c9lc00256a. Epub 2019 Jun 28.
High-throughput fluorescence polarization immunoassays (FPIAs) for mycotoxin were conducted using a portable FP analyzer with a microdevice. Simultaneous FPIA measurements for 8 different deoxynivalenol (DON) concentrations in 12 chambers (total of 96 samples) and high-throughput FPIA measurements for single DON concentrations in more than 500 chambers were conducted. The results indicated that simultaneous FPIAs for 96 independent samples and for 500 samples were possible by FP imaging. The FP analyzer has a size of 65 cm (W 35 cm × D 15 cm × H 15 cm) and costs less than $5000. The sample volume was 1 nL. Furthermore, it is expected that sample reaction and FP detection can be automatically conducted with the analyzer by changing the microdevice and the software. Its features such as low cost and portability will contribute to on-site measurement and point-of-care testing. Additionally, the high-throughput feature will contribute to the study of molecular interactions based on FP measurements.
采用带有微器件的便携式荧光偏振免疫分析仪（FPIA）进行真菌毒素高通量荧光偏振免疫分析（FPIA）。在 12 个腔室中同时进行 8 种不同脱氧雪腐镰刀菌烯醇（DON）浓度的 FPIA 测量（共 96 个样品），并在 500 多个腔室中进行单个 DON 浓度的高通量 FPIA 测量。结果表明，通过 FP 成像可以同时进行 96 个独立样本和 500 个样本的 FPIA。该 FP 分析仪尺寸为 65cm（宽 35cm×深 15cm×高 15cm），成本低于 5000 美元。样品体积为 1nL。此外，通过更换微器件和软件，有望实现分析仪的自动样品反应和 FP 检测。其低成本和便携性等特点将有助于现场测量和即时检测。此外，高通量功能将有助于基于 FP 测量的分子相互作用研究。
