半胱氨酸-亚硫酸盐反应新见解。

New Insights into the Cystine-Sulfite Reaction.

机构信息

Life Sciences, Pharmacy and Chemistry, Kingston University, Surrey, KT1 2EE, Kingston-upon-Thames, UK.

GlaxoSmithKline, Consumer Healthcare, Weybridge, KT13 0DE, UK.

出版信息

Molecules. 2019 Jun 27;24(13):2377. doi: 10.3390/molecules24132377.

DOI:10.3390/molecules24132377

PMID:31252546

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6650951/

Abstract

The mechanism by which cysteine-S-sulfate is formed from the reaction of sulfite with cystine in the absence of a dedicated oxidizing agent is investigated using high-resolution NMR. Changes to reactant ratio, pH, UV light exposure and temperature were evaluated to determine the most effective conditions to achieve the maximum yield of cysteine-S-sulfate without recourse to conventional oxidizing reagents. Herein evidence is provided for both nucleophilic and radical mechanisms, by which cysteine-S-sulfate can be generated with yields of up to 96%.

摘要

使用高分辨率 NMR 研究了亚硫酸根与胱氨酸在没有专用氧化剂的情况下形成半胱氨酸-S-硫酸盐的反应机制。通过改变反应物比例、pH 值、紫外线照射和温度来评估最有效的条件，以在不使用传统氧化剂的情况下获得最大的半胱氨酸-S-硫酸盐产率。本文提供了亲核和自由基两种机制的证据，通过这两种机制可以生成高达 96%的半胱氨酸-S-硫酸盐。