Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China.
Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China; University of Chinese Academy of Sciences, Beijing 10049, PR China.
Comp Biochem Physiol B Biochem Mol Biol. 2019 Oct;236:110310. doi: 10.1016/j.cbpb.2019.110310. Epub 2019 Jun 27.
Myogenic regulatory factor 4 (MRF4) is a basic helix-loop-helix (bHLH) transcription factor that plays crucial roles in myoblast differentiation and maturation. Here, we report the isolation of the olive flounder (Paralichthys olivaceus) mrf4 gene and the spatiotemporal analysis of its expression patterns. Sequence analysis indicated that flounder mrf4 shared a similar structure with other vertebrate MRF4, including the conserved bHLH domain. Flounder mrf4 contains 3 exons and 2 introns. Sequence alignment and phylogenetic analysis showed that it was highly homologous with Salmo salar, Danio rerio, Takifugu rubripes, and Tetraodon nigroviridis mrf4. Flounder mrf4 was first expressed in the medial region of somites that give rise to slow muscles, and later spread to the lateral region of somites that give rise to fast muscles. Mrf4 transcript levels decreased significantly in mature somites in the trunk region, and expression could only be detected in the caudal somites, consistent with the timing of somite maturation. Transient expression analysis showed that the 506 bp flounder mrf4 promoter was sufficient to direct muscle-specific GFP expression in zebrafish embryos.
肌调节因子 4（MRF4）是一种基本螺旋-环-螺旋（bHLH）转录因子，在成肌细胞分化和成熟中发挥着关键作用。在这里，我们报道了牙鲆（Paralichthys olivaceus）mrf4 基因的分离及其表达模式的时空分析。序列分析表明，牙鲆 mrf4 与其他脊椎动物 MRF4 具有相似的结构，包括保守的 bHLH 结构域。牙鲆 mrf4 包含 3 个外显子和 2 个内含子。序列比对和系统发育分析表明，它与 Salmo salar、Danio rerio、Takifugu rubripes 和 Tetraodon nigroviridis mrf4 高度同源。牙鲆 mrf4 首先在形成慢肌的体节中部表达，然后扩散到形成快肌的体节外侧。在躯干区域成熟的体节中，Mrf4 转录本水平显著降低，只能在尾段体节中检测到表达，与体节成熟的时间一致。瞬时表达分析表明，506bp 的牙鲆 mrf4 启动子足以在斑马鱼胚胎中指导肌肉特异性 GFP 表达。
