Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, People's Republic of China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150069, People's Republic of China.
Infect Genet Evol. 2019 Nov;75:103942. doi: 10.1016/j.meegid.2019.103942. Epub 2019 Jun 27.
In the present study, a thorough comparison of the infectious bronchitis virus (IBV) TC07-2/GVI-1 linage was conducted by comparing the S1 gene sequences of GVI-1 viruses with those of viruses representing the established genotypes and lineages. IBV GVI-1 strains were found to be closely genetically related to each other, irrespective of where the viruses were isolated, and differed from other known IBV genotypes and lineages; thus, it was confirmed that GVI represents a novel genotype. However, the GVI-1 viruses exhibited variable antigenicity when compared to each other. Further analysis found that strains CO8089L and CO8091L, which were isolated in Colombia in 2003, were closely related to GVI-1 viruses, suggesting that GVI-1 viruses likely originated from Colombia and are prevalent in at least five countries (Colombia, China, the Republic of Korea, Japan, and Vietnam). Analysis of the complete GVI-1 virus genomes suggested that the GVI-1 strains in China may be independently derived from recombination events that occurred between GI-19 strains and CO8089L/CO8091L-like viruses following the introduction of the viruses from Colombia. Similar to the viruses isolated in the Republic of Korea, GVI-1 viruses isolated in China also showed an affinity for the respiratory tract of chickens, which differed from one of the deduced parental viruses, the GI-19 strain. This difference may be due to recombination events that occurred in the genomes of the GVI-1 viruses, resulting in the replacement of the spike gene sequences in an YX10-like strain of GI-19 lineage.
在本研究中，通过比较 GVI-1 病毒的 S1 基因序列与代表已建立的基因型和谱系的病毒，对传染性支气管炎病毒（IBV）TC07-2/GVI-1 谱系进行了彻底的比较。发现 GVI-1 株彼此之间在遗传上密切相关，无论病毒是在哪里分离的，并且与其他已知的 IBV 基因型和谱系不同；因此，确认 GVI 代表一种新型基因型。然而，GVI-1 病毒彼此之间表现出可变的抗原性。进一步分析发现，2003 年在哥伦比亚分离的 CO8089L 和 CO8091L 株与 GVI-1 病毒密切相关，表明 GVI-1 病毒可能起源于哥伦比亚，并且至少在五个国家（哥伦比亚、中国、韩国、日本和越南）流行。对完整的 GVI-1 病毒基因组的分析表明，中国的 GVI-1 株可能是独立衍生的，是在从哥伦比亚引入病毒后，GI-19 株和 CO8089L/CO8091L 样病毒之间发生的重组事件的结果。与在韩国分离的病毒相似，在中国分离的 GVI-1 病毒也显示出对鸡呼吸道的亲和力，与其中一个推断的亲代病毒，GI-19 株不同。这种差异可能是由于 GVI-1 病毒基因组中发生的重组事件导致 GI-19 谱系中的 YX10 样株的刺突基因序列被取代。
