Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing 400044, China.
Department of Gastroenterology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
Biomaterials. 2019 Oct;217:119300. doi: 10.1016/j.biomaterials.2019.119300. Epub 2019 Jun 23.
Bone marrow derived mesenchymal stromal cells (BMSCs) migration to injury site is a prevalent event in tissue repair process after damage occurrence. The migration process is always accompanied with matrix stiffness change. In this study, sodium alginate hydrogels with different stiffness and Transwell chambers with gradient chemical factors were employed to mimic tissue repair in vivo. In this work, in the stiffness range of 1-20 kPa, BMSCs in stiffer matrix showed higher migration speed compared to those in softer matrix. Moreover, stiffer matrix decreased the nuclear stiffness of BMSCs and reduced the expression of lamin A/C, which playing a main role in the regulation of nuclear stiffness. Furthermore, it was found that BMSCs fitted environment by selecting migration strategy. This study provides a novel platform for the investigation of BMSCs migration to mimic the natural tissue repair process.
骨髓间充质基质细胞（BMSCs）向损伤部位的迁移是损伤发生后组织修复过程中的一个普遍事件。迁移过程总是伴随着基质硬度的变化。在这项研究中，使用不同硬度的海藻酸钠水凝胶和具有梯度化学因子的 Transwell 室来模拟体内组织修复。在这项工作中，在 1-20kPa 的硬度范围内，较硬基质中的 BMSCs 比较软基质中的 BMSCs 具有更高的迁移速度。此外，较硬的基质降低了 BMSCs 的核硬度，并降低了核层粘连蛋白 A/C 的表达，核层粘连蛋白 A/C 在调节核硬度中起主要作用。此外，还发现 BMSCs 通过选择迁移策略来适应环境。这项研究为研究 BMSCs 迁移提供了一个新的平台，以模拟自然组织修复过程。
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