Molnárová M, Arendarcik J
Vysoká skola veterinárska, Kosice.
Vet Med (Praha). 1987 Nov;32(11):659-68.
The changes in the concentrations of proteolytic enzyme inhibitors after the administration of hormones influencing the activity of the ovaries can cause an imbalance in the development of follicles. Important roles are attributed to serine proteases during the development of follicle, ovulation, and activity of the corpus luteum. In order to find out the changes in the trypsin inhibiting activities (TIA) of the blood plasma and cervical mucus after the administration of PGF2 alpha (100 + 100 micrograms in January and 250 + 250 micrograms in May on the 0th and 11th day) and after the single administration of 750 I. U. PMSG on the 10th day, a 14-day trial was performed to investigate the circadian changes. TIA was determined by means of slowing down the hydrolysis of the synthetic substrate N-alpha-tosyl-p-nitro-anilide (TAPA) by bovine trypsin. When the blood plasma TIA is expressed as the percent of the control values, a large drop is observed on the 11th and 12th days of the January trial (total as well as low-molecular TIA). In the May experiment this drop was recorded only on the 11th day in total TIA (c TIA); low-molecular TIA (n TIA), on the other hand, increased, particularly after the administration of PMSG. The average values of plasma TIA's, recorded in the course of the 14-day trial, increased after the administration of PGF2 alpha (c TIA increased to 109.8 +/- 22.1%, n TIA to 135.42 +/- 54.0% in January; c TIA to 112.59 +/- 50.5%, n TIA to 109.48 +/- 55.9% in May). After the administration of PMSG the TIA values remained higher than the controls (c TIA 107.13 +/- 24.0%, n TIA 133.40 +/- 52.1% in January; c TIA 113.87 +/- 51.3%, n TIA 118.33 +/- 55.6% in May). The average TIA values of the cervical mucus increased after PGF2 alpha administration to 3.06 +/- 1.7% in comparison with the control value of 2.59 +/- 1.1%, and also after the administration of PMSG to 2.82 +/- 1.9% (January, TIA per 1 mg of mucus).
给予影响卵巢活性的激素后，蛋白水解酶抑制剂浓度的变化会导致卵泡发育失衡。丝氨酸蛋白酶在卵泡发育、排卵及黄体活性过程中发挥着重要作用。为了探究在给予前列腺素F2α（1月0日和11日各100 + 100微克，5月各250 + 250微克）后以及在第10日单次给予750国际单位孕马血清促性腺激素后，血浆和宫颈黏液中胰蛋白酶抑制活性（TIA）的变化，进行了一项为期14天的试验以研究昼夜节律变化。通过减缓牛胰蛋白酶对合成底物N-α-甲苯磺酰基-p-硝基苯胺（TAPA）的水解来测定TIA。当血浆TIA以对照值的百分比表示时，在1月的试验中，第11天和第12天观察到大幅下降（总TIA以及低分子TIA）。在5月的实验中，仅在第11天记录到总TIA（c TIA）下降；另一方面，低分子TIA（n TIA）增加，尤其是在给予孕马血清促性腺激素之后。在为期14天的试验过程中记录的血浆TIA平均值，在给予前列腺素F2α后有所增加（1月时，c TIA增加到109.8 +/- 22.1%，n TIA增加到135.42 +/- 54.0%；5月时，c TIA增加到112.59 +/- 50.5%，n TIA增加到109.48 +/- 55.9%）。给予孕马血清促性腺激素后，TIA值仍高于对照值（1月时，c TIA为107.13 +/- 24.0%，n TIA为133.40 +/- 52.1%；5月时，c TIA为113.87 +/- 51.3%，n TIA为118.33 +/- 55.6%）。与对照值2.59 +/- 1.1%相比，给予前列腺素F2α后宫颈黏液的平均TIA值增加到3.06 +/- 1.7%，给予孕马血清促性腺激素后也增加到2.82 +/- 1.9%（1月，每毫克黏液的TIA）。
