Zhang Jianjun, Liu Fengli
Department of Gastroenterology, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China.
Department of Neonatal Surgery, Xuzhou Children's Hospital, Xuzhou Medical University, Xuzhou, Jiangsu 221000, P.R. China.
Exp Ther Med. 2019 Jul;18(1):225-229. doi: 10.3892/etm.2019.7530. Epub 2019 Apr 25.
Expression and clinical significance of bone morphogenetic protein (BMP)-4 and Smad1 in patients with Hirschsprung disease (HD) were investigated. A retrospective analysis of 96 HD patients (experimental group) admitted to Xuzhou Children's Hospital, Xuzhou Medical University from June 2015 to June 2017 was performed. According to the samples, the experimental group was divided into the stenosis group, the transition group and the expansion group. Forty-seven children with colostomy due to intestinal obstruction were selected as the control group. The expression levels of BMP-4 and Smad1 proteins were detected by immunohistochemical staining. The expression levels of BMP-4 and Smad mRNA were detected by real-time quantitative PCR (RT-qPCR), and were quantified and compared. Via immunohistochemistry, BMP-4 and Smad1 proteins were detected in the samples of different parts of HD patients and children with intestinal obstruction. The positive expression levels of BMP-4 and Smad1 proteins in the transition group were decreased compared with those in the expansion and control groups (P<0.05), and the positive expression levels of BMP-4 and Smad1 proteins in the stenosis group were decreased compared with those in the transition, expansion, and control groups (P<0.05). Also, the gene expression levels of BMP-4 and Smad1 in the transition and stenosis groups were successively decreased, and the differences were statistically significant (P<0.05). In conclusion, the expression of BMP-4 and Smad1 in the intestinal plexus of HD lesions was significantly reduced, indicating that BMP-4 and Smad1 are closely related to the occurrence of HD, and it is suspected that they have a certain influence on the intestinal development of congenital digestive tract malformations.
研究了骨形态发生蛋白（BMP）-4和Smad1在先天性巨结肠症（HD）患者中的表达及临床意义。对2015年6月至2017年6月在徐州医科大学附属徐州儿童医院收治的96例HD患者（实验组）进行回顾性分析。根据样本，实验组分为狭窄组、移行组和扩张组。选取47例因肠梗阻行结肠造口术的患儿作为对照组。采用免疫组织化学染色检测BMP-4和Smad1蛋白的表达水平。采用实时定量PCR（RT-qPCR）检测BMP-4和Smad mRNA的表达水平，并进行定量比较。通过免疫组织化学方法，在HD患者和肠梗阻患儿不同部位的样本中检测到BMP-4和Smad1蛋白。移行组BMP-4和Smad1蛋白的阳性表达水平低于扩张组和对照组（P<0.05），狭窄组BMP-4和Smad1蛋白的阳性表达水平低于移行组、扩张组和对照组（P<0.05）。此外，移行组和狭窄组中BMP-4和Smad1的基因表达水平依次降低，差异具有统计学意义（P<0.05）。综上所述，HD病变肠丛中BMP-4和Smad1的表达明显降低，提示BMP-4和Smad1与HD的发生密切相关，推测它们对先天性消化道畸形的肠道发育有一定影响。
