Laboratorio de Neurofarmacología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, México.
Unidad Periférica Xalapa, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Instituto de Neuroetología Universidad Veracruzana, Xalapa, México.
Acta Pharm. 2018 Sep 1;68(3):381-388. doi: 10.2478/acph-2018-0027.
The present study investigated the sensitivity to stress and diazepam in weaning (21-day old) Wistar rats. A single 15-min session of forced swimming was used to induce anxiety-like behavior. The group that was forced to swim exhibited an increase in anxiety-like behavior in the elevated plus maze (EPM) and open field test (OFT) compared to the non-stressed group. Diazepam (1 h before the tests) reduced anxiety-like behavior in rats forced to swim compared to the vehicle stressed group. The dose-response curve for diazepam indicated that the 0.5 mg kg-1 dose (1 h before the EPM and OFT) was the minimum effective dose in reducing anxiety-like behavior without altering locomotor activity in weaning rats. These results indicate that weaning rats can develop anxiety-like behavior after a brief, single session of stress, and that rats at this age are seemingly more sensitive to diazepam than adult rats, which may be taken into account for clinical applications.
本研究调查了断奶（21 日龄）Wistar 大鼠对压力和地西泮的敏感性。单次 15 分钟的强迫游泳被用于诱导焦虑样行为。与非应激组相比，被迫游泳的组在高架十字迷宫（EPM）和旷场试验（OFT）中表现出焦虑样行为增加。地西泮（在测试前 1 小时）与应激的载体组相比，减少了被迫游泳的大鼠的焦虑样行为。地西泮的剂量反应曲线表明，在 EPM 和 OFT 前 0.5mgkg-1 的剂量（1 小时）是减少焦虑样行为的最小有效剂量，而不会改变断奶大鼠的运动活性。这些结果表明，短暂的单次应激后，断奶大鼠可能会出现焦虑样行为，而且这个年龄段的大鼠对地西泮似乎比成年大鼠更敏感，这可能在临床应用中需要考虑。
