Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, P.R. China.
University of Chinese Academy of Sciences, Beijing 100049, P.R. China.
FEMS Microbiol Ecol. 2019 Aug 1;95(8). doi: 10.1093/femsec/fiz100.
The rhizospheric microbiome appears to be one of the key determinants of plant health and productivity. In this study, to understand the assembly process of the rhizospheric microbiome, the effects of different sites, soils and plants on the rhizospheric microbiome were compared and examined using high-throughput sequencing. A series of comparisons of rhizospheric microbiomes were conducted using two plants (wild rice (Oryza rufipogon Griff.) and L. hexandra (Leersia hexandra Swartz)), two soils (high nutrient and low nutrient) and two sites (Guangdong and Hainan provinces in China). The results of the redundancy analysis, between-class analysis and coinertia analysis indicated that the factors shaping the rhizospheric microbiome (in decreasing order of strength), were the site, soil and plant. The effects of plants on the rhizospheric microbiome were slight and unobvious, with relatively low-explained variations and few core groups and indicator groups; however, the effects were significant across different sites and soils, especially for specific microbial groups that are closely associated with plants, such as pathogens, symbionts, and saprotrophs. Furthermore, rhizospheric fungi were more strongly influenced by plants than rhizospheric bacteria. Our results provide insights into the relationships among multiple factors that shape the rhizospheric microbiome in natural ecosystems and highlight the effects of plants across regional environmental shifts.
根际微生物组似乎是植物健康和生产力的关键决定因素之一。在这项研究中，为了了解根际微生物组的组装过程，我们使用高通量测序比较和研究了不同地点、土壤和植物对根际微生物组的影响。我们使用两种植物（野生稻（Oryza rufipogon Griff.）和旱禾（Leersia hexandra Swartz））、两种土壤（高营养和低营养）和两个地点（中国的广东和海南）对根际微生物组进行了一系列比较。冗余分析、分类间分析和共协分析的结果表明，塑造根际微生物组的因素（按强度递减顺序）依次为地点、土壤和植物。植物对根际微生物组的影响很小且不明显，解释的变化相对较低，核心组和指示组较少；然而，这种影响在不同的地点和土壤中是显著的，特别是对于与植物密切相关的特定微生物群体，如病原体、共生体和腐生体。此外，根际真菌比根际细菌受植物的影响更大。我们的研究结果深入了解了自然生态系统中塑造根际微生物组的多种因素之间的关系，并强调了植物在区域环境变化中的作用。
