基于分子印迹聚合物纳米粒子的方法测定玉米中的伏马菌素 B1。
Determination of Fumonisin B1 in maize using molecularly imprinted polymer nanoparticles-based assay.
机构信息
Biotechnology Group, Department of Chemistry, University of Leicester, Leicester, United Kingdom; Indonesian Research Centre for Veterinary Science, IAARD-Ministry of Agriculture, Bogor, Indonesia.
Biotechnology Group, Department of Chemistry, University of Leicester, Leicester, United Kingdom; Department of Chemistry, College of Science, Mustansiriyah University, Baghdad, Iraq.
出版信息
Food Chem. 2019 Nov 15;298:125044. doi: 10.1016/j.foodchem.2019.125044. Epub 2019 Jun 19.
Fumonisin B1 (FB1) is a carcinogenic mycotoxin produced by Fusarium species contaminating maize. At present, fumonisin determination is performed using costly and demanding chromatography techniques or immunoassays. Recently, a molecularly imprinted polymer nanoparticles (nanoMIPs) - based assay (MINA) has been developed for FB1 detection. Herein, we have applied MINA for the determination of FB1 in naturally contaminated maize samples and results were compared with those obtained with ELISA and a reference HPLC method (AOAC No. 2001.04). The nanoMIPs as a recognition element mimicking antibodies used in ELISA were produced by solid phase synthesis and used in MINA for FB1 determination in 53 maize samples. As a result, 18 maize samples were contaminated with FB1 at levels higher than 0.25 mg/kg. Fumonisin concentrations from samples measured by MINA were well correlated with those using ELISA and HPLC. Therefore, MINA could be used as an alternative technique for FB1 determination in maize.
伏马菌素 B1（FB1）是一种由镰刀菌属污染玉米产生的致癌真菌毒素。目前，伏马菌素的测定采用昂贵且要求苛刻的色谱技术或免疫测定法。最近，已经开发出一种基于分子印迹聚合物纳米粒子（nanoMIPs）的测定法（MINA）用于 FB1 的检测。本文将 MINA 应用于天然污染玉米样品中 FB1 的测定，并将结果与 ELISA 和参考 HPLC 方法（AOAC No. 2001.04）的结果进行比较。作为模拟 ELISA 中使用的抗体的识别元件的 nanoMIPs 通过固相合成制备，并用于 MINA 中 53 个玉米样品中 FB1 的测定。结果，18 个玉米样品中 FB1 的污染水平高于 0.25mg/kg。MINA 测定的样品中的伏马菌素浓度与 ELISA 和 HPLC 的测定结果密切相关。因此，MINA 可以用作玉米中 FB1 测定的替代技术。
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